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Carbon Ion Beams Are Effective in Targeting Cholangiocarcinoma Stem-Like Cells In Vitro and In Vivo
https://repo.qst.go.jp/records/65055
https://repo.qst.go.jp/records/6505578d3b9d0-b2ec-4499-a4a2-6313674ff844
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-06-24 | |||||
| タイトル | ||||||
| タイトル | Carbon Ion Beams Are Effective in Targeting Cholangiocarcinoma Stem-Like Cells In Vitro and In Vivo | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Wakai, Toshifumi× Yamada, Shigeru× Kamada, Tadashi× 崔 星× 山田 滋× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To examine whether carbon ion beams are effective in targeting putative human cholangicarcinoma cancer stem-like cells. Methods: Putative cancer stem cells sorted from HuccT1 and Huh28 cells were treated with or without carbon ion or X-ray irradiation and then colony and spheroid formation assay, FACS analysis, gamma-H2AX foci assay, as well as in vivo xenograft tumor control analysis were performed. Results: FACS analysis showed that a proportion of CD133+/CD90+, CD44+/ESA+ was more significantly enriched by X-rays compared to carbon-ion beams. Colony and spheroid formation assay as well as in vivo tumorigenic assay showed that CD133+/CD90+ and CD44+/ESA+ cells exactly have cancer stem cell properties. Analysis of cell survival fractions showed that carbon beams were more effective at killing those of cancer stem-like cells compared to X-rays. A more large number and large-sized gamma-H2AX foci were observed in CD44+/ESA+ cells after 24 h carbon ion beam irradiation compared to X-rays. Xenograft tumor appeared to be very resistant to X-rays, since the tumor was not controlled even treated with 60 Gy of X-rays, but it was destroyed with 35 Gy of carbon ion beam. Conclusion: In conclusion, carbon ion beams induce prolonged and complex DNA damage, therefore potentially have advantage over X-rays by targeting putative cholangiocarcinoma stem cells. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Heavy ion Therapy and Space Radiation Symposium (HITSRS) | |||||
| 発表年月日 | ||||||
| 日付 | 2013-05-18 | |||||
| 日付タイプ | Issued | |||||
