{"created":"2023-05-15T14:47:26.670186+00:00","id":65055,"links":{},"metadata":{"_buckets":{"deposit":"380153b5-3736-4aea-ad02-092fa5e68e39"},"_deposit":{"created_by":1,"id":"65055","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"65055"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00065055","sets":["10:29"]},"author_link":["641093","641091","641090","641095","641094","641092","641089"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-05-18","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: To examine whether carbon ion beams are effective in targeting putative human cholangicarcinoma cancer stem-like cells.\nMethods:  Putative cancer stem cells sorted from HuccT1 and Huh28 cells were treated with or without carbon ion or X-ray irradiation and then colony and spheroid formation assay, FACS analysis, gamma-H2AX foci assay, as well as in vivo xenograft tumor control analysis were performed. \nResults: FACS analysis showed that a proportion of CD133+/CD90+, CD44+/ESA+ was more significantly enriched by X-rays compared to carbon-ion beams. Colony and spheroid formation assay as well as in vivo tumorigenic assay showed that CD133+/CD90+ and CD44+/ESA+ cells exactly have cancer stem cell properties. Analysis of cell survival fractions showed that carbon beams were more effective at killing those of cancer stem-like cells compared to X-rays. A more large number and large-sized gamma-H2AX foci were observed in CD44+/ESA+ cells after 24 h carbon ion beam irradiation compared to X-rays. Xenograft tumor appeared to be very resistant to X-rays, since the tumor was not controlled even treated with 60 Gy of X-rays, but it was destroyed with 35 Gy of carbon ion beam. \nConclusion: In conclusion, carbon ion beams induce prolonged and complex DNA damage, therefore potentially have advantage over X-rays by targeting putative cholangiocarcinoma stem cells.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"Heavy ion Therapy and Space Radiation Symposium (HITSRS)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"641089","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakai, Toshifumi"}],"nameIdentifiers":[{"nameIdentifier":"641090","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"641091","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"641092","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"641093","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"641094","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"641095","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Carbon Ion Beams Are Effective in Targeting Cholangiocarcinoma Stem-Like Cells In Vitro and In Vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Carbon Ion Beams Are Effective in Targeting Cholangiocarcinoma Stem-Like Cells In Vitro and In Vivo"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-06-24"},"publish_date":"2013-06-24","publish_status":"0","recid":"65055","relation_version_is_last":true,"title":["Carbon Ion Beams Are Effective in Targeting Cholangiocarcinoma Stem-Like Cells In Vitro and In Vivo"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:04:34.759217+00:00"}