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Parg deficiency sensitizes to radiation of low-high LET with enhanced apoptosis induction in mouse ES cells
https://repo.qst.go.jp/records/64342
https://repo.qst.go.jp/records/64342ff7c494f-dd4d-42e0-8cc6-730f601f8d5a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-10-06 | |||||
| タイトル | ||||||
| タイトル | Parg deficiency sensitizes to radiation of low-high LET with enhanced apoptosis induction in mouse ES cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shirai, Hidenori
× Shirai, Hidenori× Hirai, Takahisa× Sasai, Keisuke× Okayasu, Ryuichi× Masutani, Mitsuko× 白井 秀徳× 平井 崇久× 笹井 啓資× 岡安 隆一× 益谷 美都子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Poly(ADP-ribose) glycohydrolase (Parg) is the main enzyme for poly(ADP-ribose) degradation. The impact of Parg and poly(ADP-ribose) polymerase-1 (Parp-1) deficiency for sensitization of low to high linear-energy-transfer (LET) radiation was studied in mouse ES cells. Parg-/- cells showed higher sensitivity to -irradiation compared to Parp-1-/- cells. Augmented H2AX levels in Parg-/- ES cells implied a delayed repair of double strand breaks. Parg-/- ES cells show increased apoptotic ladder formation after irradiation. The effect of Parg and Parp-1 deficiency on responses to heavy ion irradiation of carbon- and Fe-ions was investigated. Parg-/- and Parp-1-/- ES cells showed enhanced apoptosis 24 hrs after carbon-ion irradiation. Parg-/- cells showed sensitization after 5 Gy carbon-ion irradiation in clonogenic survival assay, whereas clear sensitization was not noticed for Parp-1-/- ES cells. Notably, sensitization was observed at higher dose than 2 Gy for both LET 13 and 70 carbon-radiation. These results suggests that Parg deficiency sensitized mouse ES cells to radiation of a wide therapeutic range of LET values with enhanced apoptotic cell death induction. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第70回日本癌学会学術総会 | |||||
| 発表年月日 | ||||||
| 日付 | 2011-10-05 | |||||
| 日付タイプ | Issued | |||||
