@misc{oai:repo.qst.go.jp:00064342,
 author = {Shirai, Hidenori and Hirai, Takahisa and Sasai, Keisuke and Okayasu, Ryuichi and Masutani, Mitsuko and 白井 秀徳 and 平井 崇久 and 笹井 啓資 and 岡安 隆一 and 益谷 美都子},
 month = {Oct},
 note = {Poly(ADP-ribose) glycohydrolase (Parg) is the main enzyme for poly(ADP-ribose) degradation. The impact of Parg and poly(ADP-ribose) polymerase-1 (Parp-1) deficiency for sensitization of low to high linear-energy-transfer (LET) radiation was studied in mouse ES cells. Parg-/- cells showed higher sensitivity to -irradiation compared to Parp-1-/- cells. Augmented H2AX levels in Parg-/- ES cells implied a delayed repair of double strand breaks. Parg-/- ES cells show increased apoptotic ladder formation after irradiation. The effect of Parg and Parp-1 deficiency on responses to heavy ion irradiation of carbon- and Fe-ions was investigated. Parg-/- and Parp-1-/- ES cells showed enhanced apoptosis 24 hrs after carbon-ion irradiation. Parg-/- cells showed sensitization after 5 Gy carbon-ion irradiation in clonogenic survival assay, whereas clear sensitization was not noticed for Parp-1-/- ES cells. Notably, sensitization was observed at higher dose than 2 Gy for both LET 13 and 70 carbon-radiation. 
These results suggests that Parg deficiency sensitized mouse ES cells to radiation of a wide therapeutic range of LET values with enhanced apoptotic cell death induction., 第70回日本癌学会学術総会},
 title = {Parg deficiency sensitizes to radiation of low-high LET with enhanced apoptosis induction in mouse ES cells},
 year = {2011}
}