WEKO3
アイテム
A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients.
https://repo.qst.go.jp/records/64252
https://repo.qst.go.jp/records/64252ae92e033-394a-45f5-8f30-7b0721887b70
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-06-13 | |||||
| タイトル | ||||||
| タイトル | A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshii, Yukie
× Yoshii, Yukie× Yoneda, Makoto× Ikawa, Masamichi× Furukawa, Takako× Kiyono, Yashushi× Mori, Tetsuya× Yoshii, Hiroshi× Oyama, Nobuyuki× Okazawa, Hidehiko× Fujibayashi, Yasuhisa× 吉井 幸恵× 古川 高子× 吉井 裕× 藤林 康久 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: Radiolabeled Cu-diacetyl-bis (N4-methylthiosemicarbazone) (*Cu-ATSM), including 60/62/64Cu-ATSM, is a potential imaging agent of hypoxic tumors for PET. We have reported that *Cu-ATSM is trapped in tumor cells under intracellular over-reduced states eg. hypoxia. Here we evaluated *Cu-ATSM as an indicator of intercellular over-reduced states in disorders with mitochondrial dysfunction. Methods: Mitochondrial DNA-less 0 cells and the parental 143B human osteosarcoma cells, and the cybrids carrying mutated mitochondria from patients of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) (2SD cells) and that carrying wild-type one (2SA) were used. Cells were treated under normoxia or hypoxia and 64Cu-ATSM uptake was examined to compare it with levels of biological reductant NADH and NADPH. Results: 0 206 cells showed 2.7-fold higher 64Cu-ATSM uptake than parent 143B cells under normoxia (P<0.05), whereas the 64Cu-ATSM uptake was not increased under hypoxia. The NADH and NADPH levels showed correlate change to 64Cu-ATSM uptake, but it was not increased under hypoxia. 2SD cells showed 1.5-fold increase in 64Cu-ATSM uptake under normoxia as compared with 2SA, whereas 2SD cells showed no significant increased 64Cu-ATSM uptake under hypoxia, which was correlated with NADH and NADPH levels. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ISNM 2011 Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2011-06-08 | |||||
| 日付タイプ | Issued | |||||
