{"created":"2023-05-15T14:46:50.004680+00:00","id":64252,"links":{},"metadata":{"_buckets":{"deposit":"6b0fcd1f-1fa0-4067-867b-2f6e8a3cc5da"},"_deposit":{"created_by":1,"id":"64252","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64252"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064252","sets":["10:29"]},"author_link":["633671","633665","633667","633668","633676","633678","633674","633672","633666","633677","633670","633673","633669","633675"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-06-08","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objectives: Radiolabeled Cu-diacetyl-bis (N4-methylthiosemicarbazone) (*Cu-ATSM), including 60/62/64Cu-ATSM, is a potential imaging agent of hypoxic tumors for PET. We have reported that *Cu-ATSM is trapped in tumor cells under intracellular over-reduced states eg. hypoxia. Here we evaluated *Cu-ATSM as an indicator of intercellular over-reduced states in disorders with mitochondrial dysfunction. Methods: Mitochondrial DNA-less &#61554;0 cells&#61472; and the parental 143B human osteosarcoma cells, and the cybrids carrying mutated mitochondria from patients of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) (2SD cells) and that carrying wild-type one (2SA) were used. Cells were treated under normoxia or hypoxia and 64Cu-ATSM uptake was examined to compare it with levels of biological reductant NADH and NADPH. Results: &#61554;0 206 cells showed 2.7-fold higher 64Cu-ATSM uptake than parent 143B cells under normoxia (P<0.05), whereas the 64Cu-ATSM uptake was not increased under hypoxia. The NADH and NADPH levels showed correlate change to 64Cu-ATSM uptake, but it was not increased under hypoxia. 2SD cells showed 1.5-fold increase in 64Cu-ATSM uptake under normoxia as compared with 2SA, whereas 2SD cells showed no significant increased 64Cu-ATSM uptake under hypoxia, which was correlated with NADH and NADPH levels.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"ISNM　2011　Annual Meeting","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshii, Yukie"}],"nameIdentifiers":[{"nameIdentifier":"633665","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoneda, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"633666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikawa, Masamichi"}],"nameIdentifiers":[{"nameIdentifier":"633667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"633668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kiyono, Yashushi"}],"nameIdentifiers":[{"nameIdentifier":"633669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"633670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshii, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"633671","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oyama, Nobuyuki"}],"nameIdentifiers":[{"nameIdentifier":"633672","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazawa, Hidehiko"}],"nameIdentifiers":[{"nameIdentifier":"633673","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujibayashi, Yasuhisa"}],"nameIdentifiers":[{"nameIdentifier":"633674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉井 幸恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"633675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"633676","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉井 裕","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"633677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤林 康久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"633678","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients."}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-06-13"},"publish_date":"2011-06-13","publish_status":"0","recid":"64252","relation_version_is_last":true,"title":["A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:13:44.247904+00:00"}