@misc{oai:repo.qst.go.jp:00064252,
 author = {Yoshii, Yukie and Yoneda, Makoto and Ikawa, Masamichi and Furukawa, Takako and Kiyono, Yashushi and Mori, Tetsuya and Yoshii, Hiroshi and Oyama, Nobuyuki and Okazawa, Hidehiko and Fujibayashi, Yasuhisa and 吉井 幸恵 and 古川 高子 and 吉井 裕 and 藤林 康久},
 month = {Jun},
 note = {Objectives: Radiolabeled Cu-diacetyl-bis (N4-methylthiosemicarbazone) (*Cu-ATSM), including 60/62/64Cu-ATSM, is a potential imaging agent of hypoxic tumors for PET. We have reported that *Cu-ATSM is trapped in tumor cells under intracellular over-reduced states eg. hypoxia. Here we evaluated *Cu-ATSM as an indicator of intercellular over-reduced states in disorders with mitochondrial dysfunction. Methods: Mitochondrial DNA-less &#61554;0 cells&#61472; and the parental 143B human osteosarcoma cells, and the cybrids carrying mutated mitochondria from patients of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) (2SD cells) and that carrying wild-type one (2SA) were used. Cells were treated under normoxia or hypoxia and 64Cu-ATSM uptake was examined to compare it with levels of biological reductant NADH and NADPH. Results: &#61554;0 206 cells showed 2.7-fold higher 64Cu-ATSM uptake than parent 143B cells under normoxia (P<0.05), whereas the 64Cu-ATSM uptake was not increased under hypoxia. The NADH and NADPH levels showed correlate change to 64Cu-ATSM uptake, but it was not increased under hypoxia. 2SD cells showed 1.5-fold increase in 64Cu-ATSM uptake under normoxia as compared with 2SA, whereas 2SD cells showed no significant increased 64Cu-ATSM uptake under hypoxia, which was correlated with NADH and NADPH levels., ISNM　2011　Annual Meeting},
 title = {A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients.},
 year = {2011}
}