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On radiation-induced adaptive response in fetal mice: I.. A comparative microarray analysis of gene expressions in mouse fetuses
https://repo.qst.go.jp/records/62773
https://repo.qst.go.jp/records/62773029ca1ff-af86-466e-b5bb-c2cf586a7d4b
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-10-09 | |||||
| タイトル | ||||||
| タイトル | On radiation-induced adaptive response in fetal mice: I.. A comparative microarray analysis of gene expressions in mouse fetuses | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Vares, Guillaume
× Vares, Guillaume× Bing, Wang× Shang, Yi× Ohyama, Harumi× Tanaka, Kaoru× Nakajima, Tetsuo× Nenoi, Mitsuru× Hayata, Isamu× Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ× 王 冰× 尚 奕× 大山 ハルミ× 田中 薫× 中島 徹夫× 根井 充× 早田 勇 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Exposure of sublethal doses of ionizing radiation can induce protective mecanisms against a subsequent higher dose irradiation. This phenomenon, called radiation-induced adaptive response (AR), has been described in a wide range of biological models, both in vitro and in vivo. We previously demonstrated the existence of AR in mice during late organogenesis. For better understanding of molecular mechanisms underlying AR in this model, we performed a global analysis of transcriptome regulations in cells collected from whole mouse fetuses, after in utero exposure to priming irradiation. Several combinations of radiation dose and dose-rate were applied to induce or not an AR in our system. Expression levels of 14000 different genes 24 hours after irradiation were measured. While many genes were not modulated at all after radiation exposure and 166 genes were deregulated for all conditions, we identified a panel of 861 genes (so called "AR genes") showing significantly different expression ratios under AR-inducing and non AR-inducing conditions. A sub-list of 119 AR genes (so-called ARS genes) with higher discriminative characteristics was also created. We assessed the proportion of total and AR genes involved in various molecular pathways and the number of these genes for each of the GO terms. Gene enrichment analysis revealed that growth factor, translation or metallopeptidase activities (among others) were potential targets for our study. Since AR genes were involved in a variety of functions and cellular processes, we applied a functional classification algorithm which clustered genes in a limited number of functionally related groups. Our results emphasized the role of signal transduction mechanisms in the induction of AR (one-third of AR genes were directly involved in cell signaling). Previous investigations suggested the crucial role of p53 in cells's response to radiation and potentially AR; we observed that a significant proportion of AR genes were p53-related or possessed p53-binding sites. Furthermore, many AR genes were important for fetal development, like growth factors or genes involved in signaling cascades (CK2 ...). Taken together, our results present the first evidence of molecular mechanisms modulation by AR in utero, which may have potentially important consequences for further developmental processes, and could even influence tumorigenesis. The late effects occuring in mice adapted in utero, such as chromosomal aberrations or cancer initiation) therefore are a promising and interesting area for further research. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | NIRS International Symposium on Visualization of Radiation Response at the Molecular and Cellular Levels | |||||
| 発表年月日 | ||||||
| 日付 | 2007-03-13 | |||||
| 日付タイプ | Issued | |||||
