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UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide
https://repo.qst.go.jp/records/60892
https://repo.qst.go.jp/records/60892df323a76-d5a8-4ede-aa56-29178adb8662
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-03-29 | |||||
| タイトル | ||||||
| タイトル | UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
高井, 大策
× 高井, 大策× 蜂谷, みさを× 平間, 敏靖× 明石, 真言× 高井 大策× 蜂谷 みさを× 平間 敏靖× 明石 真言 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | PURPOSE: The aim of this study is to investigate further roles of reactive oxygen species (ROS) in oxidative damages of mitochondrial DNA (mtDNA) by UV-irradiation. METHODS: We introduced cDNAs of MnSOD (manganese superoxide dismutase) and/or catalase (CAT) into the human hepatoma cell line, SK-HEP1. After UV-irradiation, purified mtDNA was hydrolyzed and analyzed using HPLC system to determine contents of 8-hydroxy-2'-deoxyguanosine (8OHdG), a marker of oxidatively damaged DNA molecule. RESULTS: UV-irradiation induced the accumulation of 8OHdG at a significant higher level in MnSOD-transfectants than in other cell lines. The accumulation of 8OHdG was reduced in cells overexpressing CAT when compared to that in the control cells. Co-overexpression of CAT inhibited the accumulation of 8OHdG in MnSOD-tranfectants and the accumulated levels of 8OHdG were in parallel with those of H2O2. CONCLUSIONS: Our results suggest that H2O2 plays a key role in mtDNA damage in UV-irradiated cells since MnSOD induces and CAT reduces the generation of H2O2. Our results also indicate that the increased activity of MnSOD may lead to a toxic effect on mtDNA by UV-irradiation. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第７６会大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2003-10-18 | |||||
| 日付タイプ | Issued | |||||
