{"created":"2023-05-15T14:44:32.424045+00:00","id":60892,"links":{},"metadata":{"_buckets":{"deposit":"4c706c97-5871-47aa-84eb-3edce0bfb197"},"_deposit":{"created_by":1,"id":"60892","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"60892"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00060892","sets":["10:29"]},"author_link":["603579","603581","603582","603584","603580","603583","603577","603578"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2003-10-18","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"PURPOSE: The aim of this study is to investigate further roles of reactive oxygen species (ROS) in oxidative damages of mitochondrial DNA (mtDNA) by UV-irradiation.\nMETHODS: We introduced cDNAs of MnSOD (manganese superoxide dismutase) and/or catalase (CAT) into the human hepatoma cell line, SK-HEP1.   After UV-irradiation, purified mtDNA was hydrolyzed and analyzed using HPLC system to determine contents of 8-hydroxy-2'-deoxyguanosine (8OHdG), a marker of oxidatively damaged DNA molecule.\nRESULTS: UV-irradiation induced the accumulation of 8OHdG at a significant higher level in MnSOD-transfectants than in other cell lines. The accumulation of 8OHdG was reduced in cells overexpressing CAT when compared to that in the control cells.   Co-overexpression of CAT inhibited the accumulation of 8OHdG in MnSOD-tranfectants and the accumulated levels of 8OHdG were in parallel with those of H2O2.\nCONCLUSIONS:  Our results suggest that H2O2 plays a key role in mtDNA damage in UV-irradiated cells since MnSOD induces and CAT reduces the generation of H2O2.  Our results also indicate that the increased activity of MnSOD may lead to a toxic effect on mtDNA by UV-irradiation.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第７６会大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高井, 大策"}],"nameIdentifiers":[{"nameIdentifier":"603577","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"蜂谷, みさを"}],"nameIdentifiers":[{"nameIdentifier":"603578","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平間, 敏靖"}],"nameIdentifiers":[{"nameIdentifier":"603579","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"明石, 真言"}],"nameIdentifiers":[{"nameIdentifier":"603580","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高井 大策","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"603581","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"蜂谷 みさを","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"603582","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平間 敏靖","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"603583","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"明石 真言","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"603584","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-03-29"},"publish_date":"2005-03-29","publish_status":"0","recid":"60892","relation_version_is_last":true,"title":["UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:48:37.960900+00:00"}