@misc{oai:repo.qst.go.jp:00060892,
 author = {高井, 大策 and 蜂谷, みさを and 平間, 敏靖 and 明石, 真言 and 高井 大策 and 蜂谷 みさを and 平間 敏靖 and 明石 真言},
 month = {Oct},
 note = {PURPOSE: The aim of this study is to investigate further roles of reactive oxygen species (ROS) in oxidative damages of mitochondrial DNA (mtDNA) by UV-irradiation.
METHODS: We introduced cDNAs of MnSOD (manganese superoxide dismutase) and/or catalase (CAT) into the human hepatoma cell line, SK-HEP1.   After UV-irradiation, purified mtDNA was hydrolyzed and analyzed using HPLC system to determine contents of 8-hydroxy-2'-deoxyguanosine (8OHdG), a marker of oxidatively damaged DNA molecule.
RESULTS: UV-irradiation induced the accumulation of 8OHdG at a significant higher level in MnSOD-transfectants than in other cell lines. The accumulation of 8OHdG was reduced in cells overexpressing CAT when compared to that in the control cells.   Co-overexpression of CAT inhibited the accumulation of 8OHdG in MnSOD-tranfectants and the accumulated levels of 8OHdG were in parallel with those of H2O2.
CONCLUSIONS:  Our results suggest that H2O2 plays a key role in mtDNA damage in UV-irradiated cells since MnSOD induces and CAT reduces the generation of H2O2.  Our results also indicate that the increased activity of MnSOD may lead to a toxic effect on mtDNA by UV-irradiation., 第７６会大会},
 title = {UV- irradiation induces oxidative damage to mitochondrial DNA primarily through hydrogen peroxide},
 year = {2003}
}