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The pyrimidine (6-4) pyrimidone photoproducts cause T to G mutations in Arabidopsis
https://repo.qst.go.jp/records/56031
https://repo.qst.go.jp/records/56031974e0b13-4b12-409b-a9da-2fe688ba445e
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-04-14 | |||||
| タイトル | ||||||
| タイトル | The pyrimidine (6-4) pyrimidone photoproducts cause T to G mutations in Arabidopsis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
坂本, 綾子
× 坂本, 綾子× Yamaguchi, H.× Teranishi, M.× 坂本 綾子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To investigate the correlation between DNA damage and mutations, we irradiated Arabidopsis plants with UV-B, UV-C or gamma-rays and measured the induced DNA damage as well as mutation frequency. The Arabidopsis thaliana plants carrying UidA166G-T were irradiated with UV-B , UV-C , or gamma-rays. After irradiation, a portion of plants was harvested immediately to detect DNA damage. The remaining plants were grown for another 7-8 days, then harvested to detect somatic mutations. Cyclobutane pyrimidine dimer (CPD) and pyrimidine (6-4) pyrimidone photoproduct [(6-4) PP] are detected by enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies against CPD and (6-4) PP. The numbers of single and double strand breaks are determined by analyzing the migration of genomic DNA in the gel. As a result, we found that both 1000 Jm-2 of UVC and 3000 Jm-2 of UVB induced comparable amount of CPDs and strand breaks. By contrast, (6-4) PPs were more abundantly induced by 1,000 Jm-2 UVC than 3,000 Jm-2 of UVB. As for the frequency of reversions in somatic cell, 1,000 Jm-2 of UVC induced more mutations than 3,000 Jm-2 of UVB. These results suggest that the (6-4) PP is a major source of somatic reversions detected in the UidA166G-T marker line. Further analysis using polymerase-deficient plants will elucidate the mechanism by which UV damage induces mutations in plant cells. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2015 p. 133, 発行日 2017-03 |
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