{"created":"2023-05-15T14:40:51.334048+00:00","id":56031,"links":{},"metadata":{"_buckets":{"deposit":"265c4efc-abb8-4efd-8bff-7900c49e697a"},"_deposit":{"created_by":1,"id":"56031","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"56031"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00056031","sets":["7"]},"author_link":["573361","573360","573363","573362"],"item_10004_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"133","bibliographic_titles":[{"bibliographic_title":"QST Takasaki Annual Report 2015"}]}]},"item_10004_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the correlation between DNA damage and mutations, we irradiated Arabidopsis plants with UV-B, UV-C or gamma-rays and measured the induced DNA damage as well as mutation frequency.　The Arabidopsis thaliana plants carrying UidA166G-T were  irradiated with UV-B , UV-C , or gamma-rays.  After irradiation, a portion of plants was harvested immediately to detect DNA damage.  The remaining plants were grown for another 7-8 days, then harvested to detect somatic mutations. Cyclobutane pyrimidine dimer (CPD) and pyrimidine (6-4) pyrimidone photoproduct [(6-4) PP] are detected by enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies against CPD and (6-4) PP.  The numbers of single and double strand breaks are determined by analyzing the migration of genomic DNA in the gel.  As a result, we found that both 1000 Jm-2 of UVC and 3000 Jm-2 of UVB induced comparable amount of CPDs and strand breaks.  By contrast, (6-4) PPs were more abundantly induced by 1,000 Jm-2 UVC than 3,000 Jm-2 of UVB.  As for the frequency of reversions in somatic cell, 1,000 Jm-2 of UVC induced more mutations than 3,000 Jm-2 of UVB.  These results suggest that the (6-4) PP is a major source of somatic reversions detected in the UidA166G-T marker line.  Further analysis using polymerase-deficient plants will elucidate the mechanism by which UV damage induces mutations in plant cells.","subitem_description_type":"Abstract"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"坂本, 綾子"}],"nameIdentifiers":[{"nameIdentifier":"573360","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamaguchi, H."}],"nameIdentifiers":[{"nameIdentifier":"573361","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Teranishi, M."}],"nameIdentifiers":[{"nameIdentifier":"573362","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂本 綾子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"573363","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The pyrimidine (6-4) pyrimidone photoproducts cause T to G mutations in Arabidopsis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The pyrimidine (6-4) pyrimidone photoproducts cause T to G mutations in Arabidopsis"}]},"item_type_id":"10004","owner":"1","path":["7"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-04-14"},"publish_date":"2017-04-14","publish_status":"0","recid":"56031","relation_version_is_last":true,"title":["The pyrimidine (6-4) pyrimidone photoproducts cause T to G mutations in Arabidopsis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:42:35.868340+00:00"}