@article{oai:repo.qst.go.jp:00056031,
 author = {坂本, 綾子 and Yamaguchi, H. and Teranishi, M. and 坂本 綾子},
 journal = {QST Takasaki Annual Report 2015},
 month = {Mar},
 note = {To investigate the correlation between DNA damage and mutations, we irradiated Arabidopsis plants with UV-B, UV-C or gamma-rays and measured the induced DNA damage as well as mutation frequency.　The Arabidopsis thaliana plants carrying UidA166G-T were  irradiated with UV-B , UV-C , or gamma-rays.  After irradiation, a portion of plants was harvested immediately to detect DNA damage.  The remaining plants were grown for another 7-8 days, then harvested to detect somatic mutations. Cyclobutane pyrimidine dimer (CPD) and pyrimidine (6-4) pyrimidone photoproduct [(6-4) PP] are detected by enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies against CPD and (6-4) PP.  The numbers of single and double strand breaks are determined by analyzing the migration of genomic DNA in the gel.  As a result, we found that both 1000 Jm-2 of UVC and 3000 Jm-2 of UVB induced comparable amount of CPDs and strand breaks.  By contrast, (6-4) PPs were more abundantly induced by 1,000 Jm-2 UVC than 3,000 Jm-2 of UVB.  As for the frequency of reversions in somatic cell, 1,000 Jm-2 of UVC induced more mutations than 3,000 Jm-2 of UVB.  These results suggest that the (6-4) PP is a major source of somatic reversions detected in the UidA166G-T marker line.  Further analysis using polymerase-deficient plants will elucidate the mechanism by which UV damage induces mutations in plant cells.},
 title = {The pyrimidine (6-4) pyrimidone photoproducts cause T to G mutations in Arabidopsis},
 year = {2017}
}