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ブラッグピーク近傍の重粒子イオンを用いたイオン特異的なＤＮＡ損傷の誘発と修復
https://repo.qst.go.jp/records/55241
https://repo.qst.go.jp/records/55241b043d413-01d3-4bc9-ba7a-cda561475658
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-01-25 | |||||
| タイトル | ||||||
| タイトル | ブラッグピーク近傍の重粒子イオンを用いたイオン特異的なＤＮＡ損傷の誘発と修復 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
小西, 輝昭
× 小西, 輝昭× 竹安, 明浩× 石澤, 紗智× 山浦, 晋× 檜枝, 光太郎× 佐藤, 幸夫× 安田, 仲宏× 古澤, 佳也× 小西 輝昭× 竹安 明弘× 石澤 紗智× 山浦 晋× 檜枝 光太郎× 佐藤 幸夫× 安田 仲宏× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | HeLa cells were irradiated with Fe ions (specific energy: 0.93 MeV/u, LET: 4.79 MeV/um3) at the Medium Energy Beam Course. Irradiated cells were immuno-stained with gamma-H2AX antibody to visualize the distribution of DNA double strand break (dsb), which specifically induces phosphorylation of H2AX (gamma-H2AX). In an irradiated cell, bright and well-defined fluorescent foci were seen in the nucleus, but in a non-irradiated cell, no or few foci were detected. Cross-sectional views that were constructed with the software using 20 sliced images of a nucleus with 0.55 um interval, showed rod-like shapes of green fluorescence from top to bottom of the nucleus. These must be cross-sectional images of the gamma-H2AX column produced by ions traversing the nucleus. The correlation between the number of gamma-H2AX foci observed and average number of ion traversals through a nucleus showed 0.92 +/- 0.09. This proves that the foci of gamma-H2AX, were produced at each ion traversal. | |||||
| 書誌情報 |
放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書 巻 2002, p. 113-114, 発行日 2003-04 |
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| 出版者 | ||||||
| 出版者 | 放医研 | |||||
