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Effects of X-irradiation on embryonic stem cells-derived neural stem cells
https://repo.qst.go.jp/records/54191
https://repo.qst.go.jp/records/54191ea09776c-4d56-4364-a20e-bb1d7983775e
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-12-29 | |||||
| タイトル | ||||||
| タイトル | Effects of X-irradiation on embryonic stem cells-derived neural stem cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Isono, Mayu
× Isono, Mayu× Otsu, Masahiro× Konishi, Teruaki× Nakayama, Takashi× Inoue, Nobuo× 小西 輝昭 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The neural stem cells increase in number and differentiate into neurons, astrocytes and oligodendrocytes in developmental brain during fetal period. Radiation exposure during the fetal period leads to disturbances in development of the brain and can induce microcephaly or anencephalia. Here we investigated biological effects of the X-irradiation on the neural stem cells, prepared from mouse embryonic stem (ES) cells by the Neural Stem Sphere (NSS) method. Cultured with mitogen fibroblast growth factor-2, the neural stem cells proliferated exponentially. When the neural stem cells were exposed to various doses of X-ray, the proliferation of the neural stem cells was dose-dependently inhibited by X-irradiation and almost completely stopped by 5 Gy-radiation. Morphology of the multipolar neural stem cells was affected by the radiation, and the surviving cells after radiation were bipolar and larger than the neural stem cells. However, immunofluorescent study showed that the surviving bipolar cells were positive for nestin, and gene expression of nestin and musashi-1 was also demonstrated in the cells by RT-PCR study. These results suggested that radiation inhibits proliferation of the neural stem cells but the surviving cells after radiation maintain basic properties of the neural stem cells. |
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| 書誌情報 |
Journal of Neurochemistry 巻 110, 号 Suppl. 2, p. 25-25, 発行日 2009-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-3042 | |||||
