@inproceedings{oai:repo.qst.go.jp:00054191,
 author = {Isono, Mayu and Otsu, Masahiro and Konishi, Teruaki and Nakayama, Takashi and Inoue, Nobuo and 小西 輝昭},
 book = {Journal of Neurochemistry},
 issue = {Suppl. 2},
 month = {Sep},
 note = {The neural stem cells increase in number and differentiate into
neurons, astrocytes and oligodendrocytes in developmental brain
during fetal period. Radiation exposure during the fetal period leads
to disturbances in development of the brain and can induce
microcephaly or anencephalia. Here we investigated biological
effects of the X-irradiation on the neural stem cells, prepared from
mouse embryonic stem (ES) cells by the Neural Stem Sphere (NSS)
method. Cultured with mitogen fibroblast growth factor-2, the
neural stem cells proliferated exponentially. When the neural stem
cells were exposed to various doses of X-ray, the proliferation of the
neural stem cells was dose-dependently inhibited by X-irradiation
and almost completely stopped by 5 Gy-radiation. Morphology of
the multipolar neural stem cells was affected by the radiation, and
the surviving cells after radiation were bipolar and larger than the
neural stem cells. However, immunofluorescent study showed that
the surviving bipolar cells were positive for nestin, and gene
expression of nestin and musashi-1 was also demonstrated in the
cells by RT-PCR study. These results suggested that radiation
inhibits proliferation of the neural stem cells but the surviving cells
after radiation maintain basic properties of the neural stem cells.},
 pages = {25--25},
 title = {Effects of X-irradiation on embryonic stem cells-derived neural stem cells},
 volume = {110},
 year = {2009}
}