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Immunotargeting of Integrin α6β4 for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model.
https://repo.qst.go.jp/records/49468
https://repo.qst.go.jp/records/49468682e135d-29ef-4804-8fc5-ba205a75038c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-01-16 | |||||
| タイトル | ||||||
| タイトル | Immunotargeting of Integrin α6β4 for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
U, Winn Aung
× U, Winn Aung× Tsuji, Atsushi× Sudo, Hitomi× Sugyo, Aya× Furukawa, Takako× Ukai, Yoshinori× Kurosawa, Yoshikazu× Saga, Tsuneo× Ｕ Ｗｉｎｎ Ａｕｎｇ× 辻 厚至× 須藤 仁美× 須尭 綾× 古川 高子× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To explore suitable imaging probes for early and specific detection of pancreatic cancer, we demonstrated that a6b4 integrin is a good target and employed single-photon emission computed tomography (SPECT) or near-infrared (NIR) imaging for immunotargeting. Expression levels of a6b4 were examined by Western blotting and flow cytometry in certain human pancreatic cancer cell lines. The human cell line BxPC-3 was used for a6b4-positive and a mouse cell line, A4, was used for negative counterpart. We labeled antibody against a6b4 with Indium-111 (111In) or indocyanine green (ICG). After injection of 111In-labeled probe to tumorbearing mice, biodistribution, SPECT, autoradiography (ARG), and immunohistochemical (IHC) studies were conducted. After administration of ICG-labeled probe, in vivo and ex vivo NIR imaging and fluorescence microscopy of tumors were performed. BxPC-3 tumor showed a higher radioligand binding in SPECT and higher fluorescence intensity as well as a delay in the probe washout in NIR imaging when compared to A4 tumor. The biodistribution profile of 111In-labeled probe, ARG, and IHC confirmed the a6b4 specific binding of the probe. Here, we propose that a6b4 is a desirable target for the diagnosis of pancreatic cancer and that it could be detected by radionuclide imaging and NIR imaging using a radiolabeled or ICG-labeled a6b4 antibody. |
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| 書誌情報 |
Molecular Imaging 巻 15, p. 1-11, 発行日 2016-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 153-0121 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 27030400 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1177/1536012115624917 | |||||
