@article{oai:repo.qst.go.jp:00049468,
 author = {U, Winn Aung and Tsuji, Atsushi and Sudo, Hitomi and Sugyo, Aya and Furukawa, Takako and Ukai, Yoshinori and Kurosawa, Yoshikazu and Saga, Tsuneo and Ｕ Ｗｉｎｎ Ａｕｎｇ and 辻 厚至 and 須藤 仁美 and 須尭 綾 and 古川 高子 and 佐賀 恒夫},
 journal = {Molecular Imaging},
 month = {Dec},
 note = {To explore suitable imaging probes for early and specific detection of pancreatic cancer, we demonstrated that a6b4 integrin is a
good target and employed single-photon emission computed tomography (SPECT) or near-infrared (NIR) imaging for immunotargeting.
Expression levels of a6b4 were examined by Western blotting and flow cytometry in certain human pancreatic cancer
cell lines. The human cell line BxPC-3 was used for a6b4-positive and a mouse cell line, A4, was used for negative counterpart. We
labeled antibody against a6b4 with Indium-111 (111In) or indocyanine green (ICG). After injection of 111In-labeled probe to tumorbearing
mice, biodistribution, SPECT, autoradiography (ARG), and immunohistochemical (IHC) studies were conducted. After
administration of ICG-labeled probe, in vivo and ex vivo NIR imaging and fluorescence microscopy of tumors were performed.
BxPC-3 tumor showed a higher radioligand binding in SPECT and higher fluorescence intensity as well as a delay in the probe
washout in NIR imaging when compared to A4 tumor. The biodistribution profile of 111In-labeled probe, ARG, and IHC confirmed
the a6b4 specific binding of the probe. Here, we propose that a6b4 is a desirable target for the diagnosis of pancreatic cancer and
that it could be detected by radionuclide imaging and NIR imaging using a radiolabeled or ICG-labeled a6b4 antibody.},
 pages = {1--11},
 title = {Immunotargeting of Integrin α6β4 for Single-Photon Emission Computed Tomography and Near-Infrared Fluorescence Imaging in a Pancreatic Cancer Model.},
 volume = {15},
 year = {2016}
}