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MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast
https://repo.qst.go.jp/records/49445
https://repo.qst.go.jp/records/494454536182a-7a21-4c24-9618-0ec792c20b81
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-01-10 | |||||
| タイトル | ||||||
| タイトル | MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Luo, Di
× Luo, Di× Kato, Daiki× Nogami, Jumpei× Ohkawa, Yasuyuki× Kurumizaka, Hitoshi× Kono, Hidetoshi× ルオ ディ× 河野 秀俊 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The first nucleosome in the downstream of transcription start site is called +1 nucleosome, which is expected to be readily unwrapped for DNA transcription. To investigate the DNA accessibility of +1 nucleosomes, MNase-seq experiments were carried out with 20 reconstituted +1 nucleosomes of yeast. We find that MNase not only cleaves the presumably accessible end regions but also the further inward sites where AA/TT dinucleotide is abundant. Since AA/TT is known as a rigid dinucleotide step resistant to DNA bending, these internal cleavages reflect the local site exposures induced by the physicochemical nature of DNA sequence. As the DNA entry site of +1 nucleosomes in yeast is found AA/TT-rich, we propose that this sequence element might be utilized by gene regulation to lower DNA-histones affinity to facilitate DNA unwrapping for transcription. Accordingly, our further analysis shows that the highly transcribed genes in yeast are more likely to position more AA/TTs in the entry-to-dyad region than the exit-to-dyad region in their +1 nucleosomes. | |||||
| 書誌情報 |
Nucleic Acids Research 巻 46, 号 14, p. 7124-7137, 発行日 2018-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0305-1048 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/nar/gky502 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1093/nar/gky502 | |||||
| 関連名称 | https://doi.org/10.1093/nar/gky502 | |||||
