{"created":"2023-05-15T14:38:18.967032+00:00","id":49445,"links":{},"metadata":{"_buckets":{"deposit":"0eb71849-4e38-4ce8-b1b4-f8853f2648ac"},"_deposit":{"created_by":1,"id":"49445","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49445"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049445","sets":["1"]},"author_link":["499373","499376","499378","499374","499379","499377","499375","499372"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"14","bibliographicPageEnd":"7137","bibliographicPageStart":"7124","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"Nucleic Acids Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The first nucleosome in the downstream of transcription start site is called +1 nucleosome, which is expected to be readily unwrapped for DNA transcription. To investigate the DNA accessibility of +1 nucleosomes, MNase-seq experiments were carried out with 20 reconstituted +1 nucleosomes of yeast. We find that MNase not only cleaves the presumably accessible end regions but also the further inward sites where AA/TT dinucleotide is abundant. Since AA/TT is known as a rigid dinucleotide step resistant to DNA bending, these internal cleavages reflect the local site exposures induced by the physicochemical nature of DNA sequence. As the DNA entry site of +1 nucleosomes in yeast is found AA/TT-rich, we propose that this sequence element might be utilized by gene regulation to lower DNA-histones affinity to facilitate DNA unwrapping for transcription. Accordingly, our further analysis shows that the highly transcribed genes in yeast are more likely to position more AA/TTs in the entry-to-dyad region than the exit-to-dyad region in their +1 nucleosomes.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/nar/gky502","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.1093/nar/gky502"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/nar/gky502","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0305-1048","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Luo, Di"}],"nameIdentifiers":[{"nameIdentifier":"499372","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kato, Daiki"}],"nameIdentifiers":[{"nameIdentifier":"499373","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nogami, Jumpei"}],"nameIdentifiers":[{"nameIdentifier":"499374","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohkawa, Yasuyuki"}],"nameIdentifiers":[{"nameIdentifier":"499375","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kurumizaka, Hitoshi"}],"nameIdentifiers":[{"nameIdentifier":"499376","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono, Hidetoshi"}],"nameIdentifiers":[{"nameIdentifier":"499377","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ルオ ディ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"499378","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河野 秀俊","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"499379","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-01-10"},"publish_date":"2019-01-10","publish_status":"0","recid":"49445","relation_version_is_last":true,"title":["MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:19:42.463739+00:00"}