@article{oai:repo.qst.go.jp:00049445,
 author = {Luo, Di and Kato, Daiki and Nogami, Jumpei and Ohkawa, Yasuyuki and Kurumizaka, Hitoshi and Kono, Hidetoshi and ルオ ディ and 河野 秀俊},
 issue = {14},
 journal = {Nucleic Acids Research},
 month = {Aug},
 note = {The first nucleosome in the downstream of transcription start site is called +1 nucleosome, which is expected to be readily unwrapped for DNA transcription. To investigate the DNA accessibility of +1 nucleosomes, MNase-seq experiments were carried out with 20 reconstituted +1 nucleosomes of yeast. We find that MNase not only cleaves the presumably accessible end regions but also the further inward sites where AA/TT dinucleotide is abundant. Since AA/TT is known as a rigid dinucleotide step resistant to DNA bending, these internal cleavages reflect the local site exposures induced by the physicochemical nature of DNA sequence. As the DNA entry site of +1 nucleosomes in yeast is found AA/TT-rich, we propose that this sequence element might be utilized by gene regulation to lower DNA-histones affinity to facilitate DNA unwrapping for transcription. Accordingly, our further analysis shows that the highly transcribed genes in yeast are more likely to position more AA/TTs in the entry-to-dyad region than the exit-to-dyad region in their +1 nucleosomes.},
 pages = {7124--7137},
 title = {MNase, as a probe to study the sequence-dependent site exposures in the +1 nucleosomes of yeast},
 volume = {46},
 year = {2018}
}