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Fluorescence and Autoradiographic Evaluation of Tau PET Ligand PBB3 to α-Synuclein Pathology
https://repo.qst.go.jp/records/48945
https://repo.qst.go.jp/records/48945ff7c9ffe-3ca0-48a5-8506-2f9559a83c61
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-05-11 | |||||
| タイトル | ||||||
| タイトル | Fluorescence and Autoradiographic Evaluation of Tau PET Ligand PBB3 to α-Synuclein Pathology | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Koga, Shunsuke
× Koga, Shunsuke× Ono, Maiko× Sahara, Naruhiko× Higuchi, Makoto× W., Dickson Dennis× 小野 麻衣子× 佐原 成彦× 樋口 真人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | ABSTRACT: Background: The tau PET ligand 2- ((1E,3E)-4-(6-([11C]methylamino)pyridin-3-yl)buta-1,3-dienyl) benzo[d]thiazol-6-ol ([11C]PBB3) binds to a wide range of tau pathology; however, binding property of PBB3 to non-tau inclusions remains unknown. To clarify whether [11C]PBB3 binds to a-synuclein pathology, reactivity of PBB3 was assessed by in vitro fluorescence and autoradiographic labeling of brain sections from a-synucleinopathies patients. Method: Of 10 pure Lewy body disease and 120 multiple system atrophy (MSA) cases in the Mayo Clinic brain bank, we selected 3 Lewy body disease and 4 MSA cases with a range of a-synuclein severity based on the quantitative analysis of a-synuclein burden. PBB3 fluorescence labeling, double or single immunostaining for a-synuclein and phospho-tau, Prussian blue staining, and in vitro autoradiography with [11C]PBB3 were performed for these selected samples. Results: PBB3 fluorescence labeled various asynuclein lesions including Lewy bodies, Lewy neurites, spheroids, glial cytoplasmic inclusions, and neuronal cytoplasmic inclusions. Meanwhile, autoradiographic labeling with [11C]PBB3 at 10nM demonstrated no significant binding in Lewy body disease cases. In contrast, significant autoradiographic binding of [11C]PBB3 to the striatopallidal fibers was found in 2 MSA cases, which had high densities of glial cytoplasmic inclusions without tau or iron deposits in this region. Conclusions: Given that the maximum concentration of [11C]PBB3 in human PET scans is approximately 10 nM, the present data imply that a-synuclein pathology in Lewy body disease is undetectable by [11C]PBB3-PET, whereas those in a subset of MSA cases with high densities of glial cytoplasmic inclusions could be captured by this radioligand. VC 2016 International Parkinson and Movement Disorder Society Key Words: Lewy body disease; multiple system atrophy; a-synucleinopathy; PBB3; autoradiography |
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| 書誌情報 |
Movement Disorders 巻 32, 号 6, p. 884-892, 発行日 2017-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0885-3185 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 28440890 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/mds.27013 | |||||
