{"created":"2023-05-15T14:37:56.748130+00:00","id":48945,"links":{},"metadata":{"_buckets":{"deposit":"7558577f-5b15-4720-bf8c-d23904414408"},"_deposit":{"created_by":1,"id":"48945","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48945"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048945","sets":["1"]},"author_link":["493268","493273","493269","493272","493271","493270","493275","493274"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"892","bibliographicPageStart":"884","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Movement Disorders"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ABSTRACT: Background: The tau PET ligand 2-\n((1E,3E)-4-(6-([11C]methylamino)pyridin-3-yl)buta-1,3-dienyl)\nbenzo[d]thiazol-6-ol ([11C]PBB3) binds to a wide range\nof tau pathology; however, binding property of PBB3 to\nnon-tau inclusions remains unknown. To clarify\nwhether [11C]PBB3 binds to a-synuclein pathology,\nreactivity of PBB3 was assessed by in vitro fluorescence\nand autoradiographic labeling of brain sections\nfrom a-synucleinopathies patients.\nMethod: Of 10 pure Lewy body disease and 120 multiple\nsystem atrophy (MSA) cases in the Mayo Clinic\nbrain bank, we selected 3 Lewy body disease and 4\nMSA cases with a range of a-synuclein severity based\non the quantitative analysis of a-synuclein burden.\nPBB3 fluorescence labeling, double or single immunostaining\nfor a-synuclein and phospho-tau, Prussian blue\nstaining, and in vitro autoradiography with [11C]PBB3\nwere performed for these selected samples.\nResults: PBB3 fluorescence labeled various asynuclein\nlesions including Lewy bodies, Lewy neurites,\nspheroids, glial cytoplasmic inclusions, and neuronal\ncytoplasmic inclusions. Meanwhile, autoradiographic\nlabeling with [11C]PBB3 at 10nM demonstrated no significant\nbinding in Lewy body disease cases. In contrast,\nsignificant autoradiographic binding of [11C]PBB3\nto the striatopallidal fibers was found in 2 MSA cases,\nwhich had high densities of glial cytoplasmic inclusions\nwithout tau or iron deposits in this region.\nConclusions: Given that the maximum concentration of\n[11C]PBB3 in human PET scans is approximately 10 nM,\nthe present data imply that a-synuclein pathology in\nLewy body disease is undetectable by [11C]PBB3-PET,\nwhereas those in a subset of MSA cases with high densities\nof glial cytoplasmic inclusions could be captured\nby this radioligand. VC 2016 International Parkinson and\nMovement Disorder Society\nKey Words: Lewy body disease; multiple system\natrophy; a-synucleinopathy; PBB3; autoradiography","subitem_description_type":"Abstract"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28440890","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/mds.27013","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0885-3185","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koga, Shunsuke"}],"nameIdentifiers":[{"nameIdentifier":"493268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ono, Maiko"}],"nameIdentifiers":[{"nameIdentifier":"493269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sahara, Naruhiko"}],"nameIdentifiers":[{"nameIdentifier":"493270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"493271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"W., Dickson Dennis"}],"nameIdentifiers":[{"nameIdentifier":"493272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野 麻衣子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"493273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐原 成彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"493274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"樋口 真人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"493275","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Fluorescence and Autoradiographic Evaluation of Tau PET Ligand PBB3 to α-Synuclein Pathology","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fluorescence and Autoradiographic Evaluation of Tau PET Ligand PBB3 to α-Synuclein Pathology"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-11"},"publish_date":"2018-05-11","publish_status":"0","recid":"48945","relation_version_is_last":true,"title":["Fluorescence and Autoradiographic Evaluation of Tau PET Ligand PBB3 to α-Synuclein Pathology"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:22:36.741184+00:00"}