@article{oai:repo.qst.go.jp:00048945,
 author = {Koga, Shunsuke and Ono, Maiko and Sahara, Naruhiko and Higuchi, Makoto and W., Dickson Dennis and 小野 麻衣子 and 佐原 成彦 and 樋口 真人},
 issue = {6},
 journal = {Movement Disorders},
 month = {Apr},
 note = {ABSTRACT: Background: The tau PET ligand 2-
((1E,3E)-4-(6-([11C]methylamino)pyridin-3-yl)buta-1,3-dienyl)
benzo[d]thiazol-6-ol ([11C]PBB3) binds to a wide range
of tau pathology; however, binding property of PBB3 to
non-tau inclusions remains unknown. To clarify
whether [11C]PBB3 binds to a-synuclein pathology,
reactivity of PBB3 was assessed by in vitro fluorescence
and autoradiographic labeling of brain sections
from a-synucleinopathies patients.
Method: Of 10 pure Lewy body disease and 120 multiple
system atrophy (MSA) cases in the Mayo Clinic
brain bank, we selected 3 Lewy body disease and 4
MSA cases with a range of a-synuclein severity based
on the quantitative analysis of a-synuclein burden.
PBB3 fluorescence labeling, double or single immunostaining
for a-synuclein and phospho-tau, Prussian blue
staining, and in vitro autoradiography with [11C]PBB3
were performed for these selected samples.
Results: PBB3 fluorescence labeled various asynuclein
lesions including Lewy bodies, Lewy neurites,
spheroids, glial cytoplasmic inclusions, and neuronal
cytoplasmic inclusions. Meanwhile, autoradiographic
labeling with [11C]PBB3 at 10nM demonstrated no significant
binding in Lewy body disease cases. In contrast,
significant autoradiographic binding of [11C]PBB3
to the striatopallidal fibers was found in 2 MSA cases,
which had high densities of glial cytoplasmic inclusions
without tau or iron deposits in this region.
Conclusions: Given that the maximum concentration of
[11C]PBB3 in human PET scans is approximately 10 nM,
the present data imply that a-synuclein pathology in
Lewy body disease is undetectable by [11C]PBB3-PET,
whereas those in a subset of MSA cases with high densities
of glial cytoplasmic inclusions could be captured
by this radioligand. VC 2016 International Parkinson and
Movement Disorder Society
Key Words: Lewy body disease; multiple system
atrophy; a-synucleinopathy; PBB3; autoradiography},
 pages = {884--892},
 title = {Fluorescence and Autoradiographic Evaluation of Tau PET Ligand PBB3 to α-Synuclein Pathology},
 volume = {32},
 year = {2017}
}