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Evaluation of developmental competence of vitrified-warmed early cleavage-stage embryos and their application for chimeric mouse production
https://repo.qst.go.jp/records/47306
https://repo.qst.go.jp/records/47306c0323684-4fc2-4090-a915-071f42dc2e63
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-09-16 | |||||
| タイトル | ||||||
| タイトル | Evaluation of developmental competence of vitrified-warmed early cleavage-stage embryos and their application for chimeric mouse production | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kito, Seiji
× Kito, Seiji× Noguchi, Yoshiko× Oota, Yuki× Ohhata, Tatsuya× Abe, Masumi× Shiomi, Naoko× Shiomi, Tadahiro× 鬼頭 靖司× 太田 有紀× 安倍 真澄× 塩見 尚子× 塩見 忠博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The developmental competence of in vitro cultured embryos vitrified-warmed at an early cleavage stage (2- or 4, 8-cell stage) was examined by both direct transfer into recipient animals and after in vitro manipulation for chimeric mice production using embryonic stem (ES) cells. Vitrified-warmed embryos transferred at the morulae and blastocyst stages showed fetus development comparable to control embryos, although blastocyst development of vitrified-warmed embryos was significantly slower than that of controls. When vitrified-warmed early cleavage stage embryos were used for chimeric mouse production using ES cells, 1 to 10% of the injected or aggregated embryos developed into chimeric neonates and germ-line chimeric mice were obtained from all ES cell lines. This study indicates that embryos developed in vitro from vitrified-warmed embryos have equivalent competence with unvitrified embryos irrespective of stage of vitrification and that these vitrified-warmed embryos maintain adequate viability even after in vitro manipulation such as aggregation and microinjection with ES cells. | |||||
| 書誌情報 |
Experimental Animals 巻 52, p. 179-183, 発行日 2003-05 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341-1357 | |||||
