@article{oai:repo.qst.go.jp:00047306,
 author = {Kito, Seiji and Noguchi, Yoshiko and Oota, Yuki and Ohhata, Tatsuya and Abe, Masumi and Shiomi, Naoko and Shiomi, Tadahiro and 鬼頭 靖司 and 太田 有紀 and 安倍 真澄 and 塩見 尚子 and 塩見 忠博},
 journal = {Experimental Animals},
 month = {May},
 note = {The developmental competence of in vitro cultured embryos vitrified-warmed at an early cleavage stage (2- or 4, 8-cell stage) was examined by both direct transfer into recipient animals and after in vitro manipulation for chimeric mice production using embryonic stem (ES) cells.  Vitrified-warmed embryos transferred at the morulae and blastocyst stages showed fetus development comparable to control embryos, although blastocyst development of vitrified-warmed embryos was significantly slower than that of controls.  When vitrified-warmed early cleavage stage embryos were used for chimeric mouse production using ES cells, 1 to 10% of the injected or aggregated embryos developed into chimeric neonates and germ-line chimeric mice were obtained from all ES cell lines.  This study indicates that embryos developed in vitro from vitrified-warmed embryos have equivalent competence with unvitrified embryos irrespective of stage of vitrification and that these vitrified-warmed embryos maintain adequate viability even after in vitro manipulation such as aggregation and microinjection with ES cells.},
 pages = {179--183},
 title = {Evaluation of developmental competence of vitrified-warmed early cleavage-stage embryos and their application for chimeric mouse production},
 volume = {52},
 year = {2003}
}