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Combining Carbon ion Radiotherapy and Local Injection of Alpha-galactosylceramide-pulsed Dendritic Cells Inhibits Lung Metastases in an in vivo Murine Model
https://repo.qst.go.jp/records/46761
https://repo.qst.go.jp/records/4676150344892-c7d1-4408-9eb8-a6abd1d54ff0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-05-22 | |||||
| タイトル | ||||||
| タイトル | Combining Carbon ion Radiotherapy and Local Injection of Alpha-galactosylceramide-pulsed Dendritic Cells Inhibits Lung Metastases in an in vivo Murine Model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ohkubo, Yu
× Ohkubo, Yu× Iwakawa, Mayumi× Seino, Ken-ichiro× Nakawatari, Miyako× Wada, Haruka× Kamijuku, Hajime× Nakamura, Etsuko× Nakano, Takashi× Imai, Takashi× 大久保 悠× 岩川 眞由美× 清野 研一郎× 中渡 美也子× 中村 悦子× 中野 隆史× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: Our previous report indicated that carbon ion beam irradiation upregulated immunogenic, underlining the potential clinical application of radioimmunotherapy. The antimetastatic efficacy of a local combination therapy of carbon-ion radiotherapy and immunotherapy was examined using in vivo murine model. Methods and Materials: Tumors of the mouse squamous cell carcinoma (NR-S1) cells inoculated in the leg of C3H/HeMsNrs mice were locally irradiated with a single 6 Gy dosage of carbon ions (290 MeV/nucleon, 6 cm spread-out Bragg peak). Thirty-six hours after irradiation, alpha-galactosylceramide (alpha-GalCer)-pulsed dendritic cells (DCs) were injected into the leg tumor. The effects on distant lung metastases were investigated using pathological observations and immunohistochemistry. Results: The mice in the control group, which were not irradiated and not injected with alpha-GalCerpulsed DCs, had 168 +/- 53.8 metastatic nodules in their lungs at 2 weeks. The lung metastases were significantly decreased in the mouse which received the combination therapy, and the mean number of lung metastatic nodules was 2.6 +/- 1.9 (P = 0.009). In addition, the size of each lung metastasis was significantly decreased in compared to the control group (P = 0.009). Immunohistochemistry showed that ICAM-1, which activates DCs, was highly expressed on the local tumors of the carbon ionirradiated group. The expression of S100A8 in lung tissue, a marker of the lung pre-metastatic phase, was decreased in the combination therapy group. Conclusion: The combination of carbon ion radiotherapy with the injection of alpha-GalCer-pulsed DCs into the primary tumors significantly decreased the number of lung metastases. |
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| 書誌情報 |
International Journal of Radiation Oncology Biology Physics 巻 78, 号 5, p. 1524-1531, 発行日 2010-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0360-3016 | |||||
