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Quantitative measurement of changes in calcium channel activity in vivo utilizing dynamic manganese-enhanced MRI (dMEMRI)
https://repo.qst.go.jp/records/46531
https://repo.qst.go.jp/records/46531c78184d8-5ae9-4431-a434-0202422bb459
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-04-15 | |||||
| タイトル | ||||||
| タイトル | Quantitative measurement of changes in calcium channel activity in vivo utilizing dynamic manganese-enhanced MRI (dMEMRI) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Leuze, Christoph
× Leuze, Christoph× Kimura, Yuichi× Kershaw, Jeffrey× Shibata, Sayaka× Saga, Tsuneo× Aoki, Ichio× et.al× ロイツェ クリストフ× 木村 裕一× Ｋｅｒｓｈａｗ Ｊｅｆｆｒｅｙ× 柴田 さやか× 佐賀 恒夫× 青木 伊知男 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The ability of manganese ions (Mn2+) to enter cells through calcium ion (Ca2+) channels has been used for depolarization dependent brain functional imaging with manganese-enhanced MRI (MEMRI). The purpose of this study was to quantify changes to Mn2+ uptake in rat brain using a dynamic manganese-enhanced MRI (dMEMRI) scanning protocol with the Patlak and Logan graphical analysis methods. The graphical analysis was based on a three-compartment model describing the tissue and plasma concentration of Mn. Mn2+ uptake was characterized by the total distribution volume of manganese (Mn) inside tissue (VT) and the unidirectional influx constant of Mn2+ from plasma to tissue (Ki). The measurements were performed on the anterior (APit) and posterior (PPit) parts of the pituitary gland, a region with an incomplete blood brain barrier. Modulation of Ca2+ channel activity was performed by administration of the stimulant glutamate and the inhibitor verapamil. It was found that the APit and PPit showed different Mn2+ uptake characteristics. While the influx of Mn2+ into the PPit was reversible, Mn2+ was found to be irreversibly trapped in the APit during the course of the experiment. In the PPit, an increase of Mn2+ uptake led to an increase in VT (from 2.8+-0.3 ml/cm3 to 4.6+-1.2 ml/cm3) while a decrease of Mn2+ uptake corresponded to a decrease in VT (from 2.8+-0.3 ml/cm3 to 1.4+-0.3 ml/cm3). In the APit, an increase of Mn2+ uptake led to an increase in Ki (from 0.034+-0.009 min−1 to 0.049+-0.012 min−1) while a decrease of Mn2+ uptake corresponded to a decrease in Ki (from 0.034+-0.009 min−1 to 0.019+-0.003 min−1). This work demonstrates that graphical analysis applied to dMEMRI data can quantitatively measure changes to Mn2+ uptake following modulation of neural activity. |
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| 書誌情報 |
NeuroImage 巻 60, 号 1, p. 392-399, 発行日 2011-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1053-8119 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.1016/j.neuroimage.2011.12.030 | |||||
| 関連名称 | http://dx.doi.org/10.1016/j.neuroimage.2011.12.030 | |||||
