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1 kbp 5' upstream sequence enables developmental stage-specific expressions of globin genes in the fish, medaka Oryzias latipes.
https://repo.qst.go.jp/records/46236
https://repo.qst.go.jp/records/462368e614a73-b175-45b9-a734-44e76bc03f4e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-10-11 | |||||
| タイトル | ||||||
| タイトル | 1 kbp 5' upstream sequence enables developmental stage-specific expressions of globin genes in the fish, medaka Oryzias latipes. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Maruyama, Kouichi
× Maruyama, Kouichi× Bing, Wang× Ishikawa, Yuji× Yasumasu, Shigeki× Iuchi, Ichiro× Kouichi, Maruyama× Bing, Wang× Yuuji, Ishikawa× Shigeki, Yasumasu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Hemoglobin of bony fish and higher vertebrates is a tetrameric protein constructed by 2 a- and 2 b-globins, which are expressed in a developmental stage-specific manner. The genomic organization of genes for embryonic and adult a- and b-globin varies from species to species. In fish, it is known that there is a unique genomic organization of globin genes, that is, a- and b-globin genes are arranged in a bi-directional and head-to-head orientation with respect to transcription start sites. In medaka, we have demonstrated that 14 globin genes are located in 2 different clusters, and 5 pairs of the a- and b-globin genes were found to be organized in a head-to-head orientation. The developmental expression patterns of the 11 globin genes were classified into 4 types. To clarify how their developmental stage-specific expressions are regulated, we produced 4 types of GFP- or RFP-transgenic medaka. Such transgenic medaka revealed that each of the 1-1.7 kbp 5 upstream sequences from respective globin genes possesses the ability to regulate the developmental stage-specific globin gene expression. In particular, the intervals between head-to-head a3 and b3, and a4 and b4 genes controlled the synchronized expression of the globin genes located at both sides of the intervals, which is significant to understand the mechanism by which equal amounts of a- and b-globins are expressed in erythroid cells. We also demonstrated that the head-to-head intervals can control the expression of the globin genes located at both sides. These findings are significant to understand the mechanism by which a- and b-globins are equally expressed in erythroid cells. |
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| 書誌情報 |
Gene 巻 492, 号 1, p. 212-219, 発行日 2012-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0378-1119 | |||||
