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In vivo tracking of transplanted mononuclear cells using manganese-enhanced magnetic resonance imaging (MEMRI)
https://repo.qst.go.jp/records/46197
https://repo.qst.go.jp/records/46197b5cf435a-da6f-4af7-9c4a-5e9b893f61e9
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-11-08 | |||||
| タイトル | ||||||
| タイトル | In vivo tracking of transplanted mononuclear cells using manganese-enhanced magnetic resonance imaging (MEMRI) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Odaka, Kenichi
× Odaka, Kenichi× Aoki, Ichio× Moriya, Junji× Tateno, Kaoru× Tadokoro, Hiroyuki× Kershaw, Jeffrey× Minamino, Tohru× Irie, Toshiaki× Fukumura, Toshimitsu× Komuro, Issei× Saga, Tsuneo× 小高 謙一× 青木 伊知男× 森谷 純治× 舘野 馨× 田所 裕之× Ｋｅｒｓｈａｗ Ｊｅｆｆｒｅｙ× 入江 俊章× 福村 利光× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Transplantation of mononuclear cells (MNCs) has previously been tested as a method to induce therapeutic angiogenesis to treat limb ischemia in clinical trials. Non-invasive high resolution imaging is required to track the cells and evaluate clinical relevance after cell transplantation. The hypothesis that MRI can provide in vivo detection and long-term observation of MNCs labeled with manganese contrast-agent was investigated in ischemic rat legs. Methods and Findings: The Mn-labeled MNCs were evaluated using 7-tesla high-field magnetic resonance imaging (MRI). Intramuscular transplanted Mn-labeled MNCs were visualized with MRI for at least 7 and up to 21 days after transplantation in the ischemic leg. The distribution of Mn-labeled MNCs was similar to that of 111In-labeled MNCs measured with singlephoton emission computed tomography (SPECT) and DiI-dyed MNCs with fluorescence microscopy. In addition, at 1–2 days after transplantation the volume of the site injected with intact Mn-labeled MNCs was significantly larger than that injected with dead MNCs, although the dead Mn-labeled MNCs were also found for approximately 2 weeks in the ischemic legs. The area covered by CD31-positive cells (as a marker of capillary endothelial cells) in the intact Mn-MNCs implanted site at 43 days was significantly larger than that at a site implanted with dead Mn-MNCs. Conclusions: The present Mn-enhanced MRI method enabled visualization of the transplanted area with a 150–175 mm inplane spatial resolution and allowed the migration of labeled-MNCs to be observed for long periods in the same subject. After further optimization, MRI-based Mn-enhanced cell-tracking could be a useful technique for evaluation of cell therapy both in research and clinical applications. |
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| 書誌情報 |
PLoS ONE (Online only:URL:http://www.plosone.org) 巻 6, 号 10, p. e25487-e25487, 発行日 2011-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1932-6203 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pone.0025487 | |||||
