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Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation
https://repo.qst.go.jp/records/46178
https://repo.qst.go.jp/records/46178d2783af2-9722-448e-9394-ec7b5491a0cf
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-10-07 | |||||
| タイトル | ||||||
| タイトル | Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Jin, Xiaodong
× Jin, Xiaodong× Li, Qiang× Wu, Qingfeng× Li, Ping× Matsumoto, Yoshitaka× Furusawa, Yoshiya× Gong, Li× Hao, Jifang× Dai, Zhongying× Ｊｉｎ Ｘｉａｏｄｏｎｇ× Ｗｕ Ｑｉｎｇｆｅｎｇ× Ｌｉ Ｐｉｎｇ× 松本 孔貴× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In this study, whether survivin plays a direct role in mediating high-LET radiation resistance in human hepatoma cells was investigated. Small interfering RNA (siRNA) targeting survivin mRNA was designed and transfected into human hepatoma HepG2 cells. Real-time PCR and western blotting analyses revealed that survivin expression in HepG2 cells decreased at both transcriptional and post-transcriptional levels after treatment with survivin-specific siRNA. Caspase-3 activity was determined with a microplate reader assay as well. Following exposure to high-LET carbon ions, a reduced clonogenic survival effect, increased apoptotic rates and caspase-3 activity were observed in the cells treated with the siRNA com- pared to those untreated with the siRNA. The cells with transfection of the survivin-specific siRNA also increased the level of G2/M arrest. These results suggest that survivin definitely plays a role in mediating the resistance of HepG2 cells to high-LET radiation and depressing survivin expression might be useful to improve the therapeutic efficacy of heavy ions for radioresistant solid tumors. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 52, 号 3, p. 335-341, 発行日 2011-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1269/jrr.10134 | |||||
