{"created":"2023-05-15T14:35:53.357596+00:00","id":46178,"links":{},"metadata":{"_buckets":{"deposit":"136d7772-f213-435b-b2e5-fb7ccad7b6f5"},"_deposit":{"created_by":1,"id":"46178","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46178"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046178","sets":["1"]},"author_link":["459822","459824","459819","459825","459828","459831","459821","459823","459826","459818","459829","459830","459827","459820"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"341","bibliographicPageStart":"335","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this study, whether survivin plays a direct role in mediating high-LET radiation resistance in human hepatoma cells was investigated. Small interfering RNA (siRNA) targeting survivin mRNA was designed and transfected into human hepatoma HepG2 cells. Real-time PCR and western blotting analyses revealed that survivin expression in HepG2 cells decreased at both transcriptional and post-transcriptional levels after treatment with survivin-specific siRNA. Caspase-3 activity was determined with a microplate reader assay as well. Following exposure to high-LET carbon ions, a reduced clonogenic survival effect, increased apoptotic rates and caspase-3 activity were observed in the cells treated with the siRNA com- pared to those untreated with the siRNA. The cells with transfection of the survivin-specific siRNA also increased the level of G2/M arrest. These results suggest that survivin definitely plays a role in mediating the resistance of HepG2 cells to high-LET radiation and depressing survivin expression might be useful to improve the therapeutic efficacy of heavy ions for radioresistant solid tumors.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1269/jrr.10134","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Jin, Xiaodong"}],"nameIdentifiers":[{"nameIdentifier":"459818","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Qiang"}],"nameIdentifiers":[{"nameIdentifier":"459819","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wu, Qingfeng"}],"nameIdentifiers":[{"nameIdentifier":"459820","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Ping"}],"nameIdentifiers":[{"nameIdentifier":"459821","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Yoshitaka"}],"nameIdentifiers":[{"nameIdentifier":"459822","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"459823","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gong, Li"}],"nameIdentifiers":[{"nameIdentifier":"459824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hao, Jifang"}],"nameIdentifiers":[{"nameIdentifier":"459825","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Dai, Zhongying"}],"nameIdentifiers":[{"nameIdentifier":"459826","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｊｉｎ Ｘｉａｏｄｏｎｇ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｗｕ Ｑｉｎｇｆｅｎｇ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459828","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｌｉ Ｐｉｎｇ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 孔貴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459830","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459831","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-10-07"},"publish_date":"2011-10-07","publish_status":"0","recid":"46178","relation_version_is_last":true,"title":["Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:54:56.058154+00:00"}