@article{oai:repo.qst.go.jp:00046178,
 author = {Jin, Xiaodong and Li, Qiang and Wu, Qingfeng and Li, Ping and Matsumoto, Yoshitaka and Furusawa, Yoshiya and Gong, Li and Hao, Jifang and Dai, Zhongying and Ｊｉｎ Ｘｉａｏｄｏｎｇ and Ｗｕ Ｑｉｎｇｆｅｎｇ and Ｌｉ Ｐｉｎｇ and 松本 孔貴 and 古澤 佳也},
 issue = {3},
 journal = {Journal of Radiation Research},
 month = {Apr},
 note = {In this study, whether survivin plays a direct role in mediating high-LET radiation resistance in human hepatoma cells was investigated. Small interfering RNA (siRNA) targeting survivin mRNA was designed and transfected into human hepatoma HepG2 cells. Real-time PCR and western blotting analyses revealed that survivin expression in HepG2 cells decreased at both transcriptional and post-transcriptional levels after treatment with survivin-specific siRNA. Caspase-3 activity was determined with a microplate reader assay as well. Following exposure to high-LET carbon ions, a reduced clonogenic survival effect, increased apoptotic rates and caspase-3 activity were observed in the cells treated with the siRNA com- pared to those untreated with the siRNA. The cells with transfection of the survivin-specific siRNA also increased the level of G2/M arrest. These results suggest that survivin definitely plays a role in mediating the resistance of HepG2 cells to high-LET radiation and depressing survivin expression might be useful to improve the therapeutic efficacy of heavy ions for radioresistant solid tumors.},
 pages = {335--341},
 title = {Radiosensitization by Inhibiting Survivin in Human Hepatoma HepG2 Cells to High-LET Radiation},
 volume = {52},
 year = {2011}
}