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Crucial Role of c-Myc in the Generation of Induced Pluripotent Stem Cells
https://repo.qst.go.jp/records/46147
https://repo.qst.go.jp/records/46147dbb24e98-a8a2-49b6-8eae-adb0dddb2d77
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-07-19 | |||||
| タイトル | ||||||
| タイトル | Crucial Role of c-Myc in the Generation of Induced Pluripotent Stem Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Araki, Ryoko
× Araki, Ryoko× Fujimori, Yuko× Uda, Masahiro× Nakamura, Miki× Jincho, Yuko× Tamura, Chihiro× Sunayama, Misato× Ando, Syunsuke× Sugiura, Mayumi× A, Yoshida Mitsuaki× Kasama, Yasuji× Abe, Masumi× 荒木 良子× 藤森 ゆう子× 宇田 昌広× 中村 美樹× 神長 祐子× 砂山 美里× 安藤 俊輔× 杉浦 真由美× 笠間 康次× 安倍 真澄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | c-Myc transduction has been considered previously to be non-essential for induced pluripotent stem cell (iPSC) generation. In our current study, we investigated the effects of c-Myc transduction on the generation of iPSCs from an inbred strain using a genome integration-free vector to exclude the effects of the genetic background and the genomic integration of exogenous genes. Our findings reveal a clear difference between iPSCs generated using the four defined factors including c-Myc (4F-iPSCs) and those produced withot c-Myc (3F-iPSCs). Molecular and cellular analyses did not reveal any differences between 3F- and 4F-iPSCs, as reported previously. However, a chimeric mice formation test indicated clear differences whereby few highly chimeric mice and no germline transmission was observed using 3F-iPSCs. Similar differences were also observed in the mouse line that has been widely used in iPSC studies. Furthermore, the defect in 3F-iPSCs was considerably improved by trichostatin A, a HDAC inhibitor, indicating that c-Myc plays a crucial role in iPSC generation through the control of histone acetylation. Indeed, low levels of histone acetylation were observed in 3F-iPSCs. Our results shed new light on iPSC generation mechanisms and strongly recommend c-Myc transduction for preparing high quality iPSCs. | |||||
| 書誌情報 |
Stem Cells 巻 29, 号 9, p. 1362-1370, 発行日 2011-07 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1066-5099 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/stem.685 | |||||
