@article{oai:repo.qst.go.jp:00046147,
 author = {Araki, Ryoko and Fujimori, Yuko and Uda, Masahiro and Nakamura, Miki and Jincho, Yuko and Tamura, Chihiro and Sunayama, Misato and Ando, Syunsuke and Sugiura, Mayumi and A, Yoshida Mitsuaki and Kasama, Yasuji and Abe, Masumi and 荒木 良子 and 藤森 ゆう子 and 宇田 昌広 and 中村 美樹 and 神長 祐子 and 砂山 美里 and 安藤 俊輔 and 杉浦 真由美 and 笠間 康次 and 安倍 真澄},
 issue = {9},
 journal = {Stem Cells},
 month = {Jul},
 note = {c-Myc transduction has been considered previously to be non-essential for induced pluripotent stem cell (iPSC) generation. In our current study, we investigated the effects of c-Myc transduction on the generation of iPSCs from an inbred strain using a genome integration-free vector to exclude the effects of the genetic background and the genomic integration of exogenous genes. Our findings reveal a clear difference between iPSCs generated using the four defined factors including c-Myc (4F-iPSCs) and those produced withot c-Myc (3F-iPSCs). Molecular and cellular analyses did not reveal any differences between 3F- and 4F-iPSCs, as reported previously. However, a chimeric mice formation test indicated clear differences whereby few highly chimeric mice and no germline transmission was observed using 3F-iPSCs. Similar differences were also observed in the mouse line that has been widely used in iPSC studies. Furthermore, the defect in 3F-iPSCs was considerably improved by trichostatin A, a HDAC inhibitor, indicating that c-Myc plays a crucial role in iPSC generation through the control of histone acetylation. Indeed, low levels of histone acetylation were observed in 3F-iPSCs. Our results shed new light on iPSC generation mechanisms and strongly recommend c-Myc transduction for preparing high quality iPSCs.},
 pages = {1362--1370},
 title = {Crucial Role of c-Myc in the Generation of Induced Pluripotent Stem Cells},
 volume = {29},
 year = {2011}
}