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Radiation-induced ICAM-1 Expression via TGF-B1 Pathway on Human Umbilical Vein Endothelial Cells; Comparison between X-ray and Carbon-ion Beam Irradiation
https://repo.qst.go.jp/records/46090
https://repo.qst.go.jp/records/460900502f570-0225-466d-9d64-852a676e97e8
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-06-03 | |||||
| タイトル | ||||||
| タイトル | Radiation-induced ICAM-1 Expression via TGF-B1 Pathway on Human Umbilical Vein Endothelial Cells; Comparison between X-ray and Carbon-ion Beam Irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kiyohara, Hiroki
× Kiyohara, Hiroki× Ishizaki, Yasuki× Suzuki, Yoshiyuki× Kato, Hiroyuki× Hamada, Nobuyuki× Ohno, Tatsuya× Takahashi, Takeo× Kobayashi, Yasuhiko× Nakano, Takashi× 清原 浩樹× 鈴木 義行× 加藤 弘之× 大野 達也× 高橋 健夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Adhesion of inflammatory cells to endothelial cells is regarded to be involved in the process of radiation-induced damage and fibrosis. Intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-beta1 (TGF-B1) are suggested to play important roles in this process. In this study, radiation-induced ICAM-1 expression was investigated with the use of an inhibitor of TGF-B1 receptor kinase (SB431542) and compared between the effects of X-ray and carbon-ion beam. Cell cultures of human umbilical vein endothelial cells (HUVE cells) were incubated with TGF-B1 and irradiated with 140 KV X-ray. Next, HUVE cells were irradiated with X-ray and 220 MeV carbon-ion beam with or without SB431542. Immunofluorescence analysis was used to quantify ICAM-1 expression. The expression of ICAM-1 in HUVE cells was significantly increased by the stimulation with TGF-B1. Expression of ICAM-1 was increased by X-ray and carbon-ion beam irradiation and decreased significantly with SB431542 after both irradiations. The expression of ICAM-1 increased by 2 Gy of carbon-ion beam irradiation was 6.7 fold higher than that of non-irradiated cells, while 5 Gy of X-ray irradiation increased the expression of ICAM-1 by 2.5 fold. According to the ICAM-1 expression, the effect of carbon-ion beam was about 2.2, 4.4 and 5.0 times greater than that of the same doses of X-ray irradiation (1, 2 and 5 Gy, respectively). The present results suggested that radiation-induced ICAM-1 expression on HUVE cells was, at least partially, regulated by TGF-B1. Carbon-ion beam induced significantly higher ICAM-1 expression than X-ray. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 52, 号 3, p. 287-292, 発行日 2011-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1269/jrr.10061 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/jrr/52/3/52_287/_article | |||||
| 関連名称 | http://www.jstage.jst.go.jp/article/jrr/52/3/52_287/_article | |||||
