@article{oai:repo.qst.go.jp:00046090,
 author = {Kiyohara, Hiroki and Ishizaki, Yasuki and Suzuki, Yoshiyuki and Kato, Hiroyuki and Hamada, Nobuyuki and Ohno, Tatsuya and Takahashi, Takeo and Kobayashi, Yasuhiko and Nakano, Takashi and 清原 浩樹 and 鈴木 義行 and 加藤 弘之 and 大野 達也 and 高橋 健夫},
 issue = {3},
 journal = {Journal of Radiation Research},
 month = {Feb},
 note = {Adhesion of inflammatory cells to endothelial cells is regarded to be involved in the process of radiation-induced damage and fibrosis.  Intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-beta1 (TGF-B1) are suggested to play important roles in this process.  In this study, radiation-induced ICAM-1 expression was investigated with the use of an inhibitor of TGF-B1 receptor kinase (SB431542) and compared between the effects of X-ray and carbon-ion beam.  Cell cultures of human umbilical vein endothelial cells (HUVE cells) were incubated with TGF-B1 and irradiated with 140 KV X-ray.  Next, HUVE cells were irradiated with X-ray and 220 MeV carbon-ion beam with or without SB431542.  Immunofluorescence analysis was used to quantify ICAM-1 expression.  The expression of ICAM-1 in HUVE cells was significantly increased by the stimulation with TGF-B1.  Expression of ICAM-1 was increased by X-ray and carbon-ion beam irradiation and decreased significantly with SB431542 after both irradiations.  The expression of ICAM-1 increased by 2 Gy of carbon-ion beam irradiation was 6.7 fold higher than that of non-irradiated cells, while 5 Gy of X-ray irradiation increased the expression of ICAM-1 by 2.5 fold.  According to the ICAM-1 expression, the effect of carbon-ion beam was about 2.2, 4.4 and 5.0 times greater than that of the same doses of X-ray irradiation (1, 2 and 5 Gy, respectively). The present results suggested that radiation-induced ICAM-1 expression on HUVE cells was, at least partially, regulated by TGF-B1.  Carbon-ion beam induced significantly higher ICAM-1 expression than X-ray.},
 pages = {287--292},
 title = {Radiation-induced ICAM-1 Expression via TGF-B1 Pathway on Human Umbilical Vein Endothelial Cells; Comparison between X-ray and Carbon-ion Beam Irradiation},
 volume = {52},
 year = {2011}
}