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Visible genotype sensor array
https://repo.qst.go.jp/records/45172
https://repo.qst.go.jp/records/45172a46213ff-73d3-41f3-b288-fa748fee55bc
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-05-27 | |||||
| タイトル | ||||||
| タイトル | Visible genotype sensor array | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Michikawa, Yuichi
× Michikawa, Yuichi× Suga, Tomo× Ohtsuka, Yoshimi× Matsumoto, Izumi× Ishikawa, Atsuko× Ishikawa, Kenichi× Iwakawa, Mayumi× Imai, Takashi× 道川 祐市× 菅 智× 荘司 好美× 松本 いづみ× 石川 敦子× 石川 顕一× 岩川 眞由美× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A visible sensor array system for simultaneous multiple SNP genotyping has been developed using a new plastic base with specific surface chemistry. Discrimination of SNP alleles is carried out by an allele-specific extension reaction using immobilized oligonucleotide primers. The 3'-ends of oligonucleotide primers are modified with a locked nucleic acid to enhance their efficiency in allelic discrimination. Biotin-dUTPs included in the reaction mixture are selectively incorporated into extending primer sequences and are utilized as tags for alkaline phosphatase-mediated precipitation of colored chemical substrates onto the surface of the plastic base. The visible precipitates allow immediate inspection of typing results by the naked eye and easy recording by a digital camera equipped on a commercial mobile phone. Up to four individuals can be analyzed on a single sensor array and multiple sensor arrays can be handled in a single operation. All of the reactions can be performed within one hour using conventional laboratory instruments. This visible genotype sensor array is suitable for "focused genomics" that follows "comprehensive genomics". |
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| 書誌情報 |
Sensors (Online Only URL:http://www.mdpi.org/sensors/) 巻 8, p. 2722-2735, 発行日 2008-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1424-8220 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | www.mdpi.org/sensors/papers/s8042722.pdf | |||||
| 関連名称 | www.mdpi.org/sensors/papers/s8042722.pdf | |||||
