@article{oai:repo.qst.go.jp:00045172,
 author = {Michikawa, Yuichi and Suga, Tomo and Ohtsuka, Yoshimi and Matsumoto, Izumi and Ishikawa, Atsuko and Ishikawa, Kenichi and Iwakawa, Mayumi and Imai, Takashi and 道川 祐市 and 菅 智 and 荘司 好美 and 松本 いづみ and 石川 敦子 and 石川 顕一 and 岩川 眞由美 and 今井 高志},
 journal = {Sensors (Online Only URL:http://www.mdpi.org/sensors/)},
 month = {Apr},
 note = {A visible sensor array system for simultaneous multiple SNP genotyping has
been developed using a new plastic base with specific surface chemistry. Discrimination of SNP alleles is carried out by an allele-specific extension reaction using immobilized oligonucleotide primers. The 3'-ends of oligonucleotide primers are modified with a locked nucleic acid to enhance their efficiency in allelic discrimination. Biotin-dUTPs included in the reaction mixture are selectively incorporated into extending primer sequences and are utilized as tags for alkaline phosphatase-mediated precipitation of colored chemical substrates onto the surface of the plastic base. The visible precipitates allow immediate inspection of typing results by the naked eye and easy recording by a digital camera equipped on a commercial mobile phone. Up to four individuals can be
analyzed on a single sensor array and multiple sensor arrays can be handled in a single operation. All of the reactions can be performed within one hour using conventional laboratory instruments. This visible genotype sensor array is suitable for "focused genomics" that follows "comprehensive genomics".},
 pages = {2722--2735},
 title = {Visible genotype sensor array},
 volume = {8},
 year = {2008}
}