{"created":"2023-05-15T14:34:57.508048+00:00","id":45172,"links":{},"metadata":{"_buckets":{"deposit":"122f7e6b-1497-44ae-90e8-113999bfc3ca"},"_deposit":{"created_by":1,"id":"45172","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45172"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045172","sets":["1"]},"author_link":["448665","448673","448674","448663","448676","448677","448670","448671","448667","448668","448672","448669","448666","448678","448664","448675"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-04","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"2735","bibliographicPageStart":"2722","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Sensors (Online Only URL:http://www.mdpi.org/sensors/)"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A visible sensor array system for simultaneous multiple SNP genotyping has\nbeen developed using a new plastic base with specific surface chemistry. Discrimination of SNP alleles is carried out by an allele-specific extension reaction using immobilized oligonucleotide primers. The 3'-ends of oligonucleotide primers are modified with a locked nucleic acid to enhance their efficiency in allelic discrimination. Biotin-dUTPs included in the reaction mixture are selectively incorporated into extending primer sequences and are utilized as tags for alkaline phosphatase-mediated precipitation of colored chemical substrates onto the surface of the plastic base. The visible precipitates allow immediate inspection of typing results by the naked eye and easy recording by a digital camera equipped on a commercial mobile phone. Up to four individuals can be\nanalyzed on a single sensor array and multiple sensor arrays can be handled in a single operation. All of the reactions can be performed within one hour using conventional laboratory instruments. This visible genotype sensor array is suitable for \"focused genomics\" that follows \"comprehensive genomics\".","subitem_description_type":"Abstract"}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"www.mdpi.org/sensors/papers/s8042722.pdf"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"www.mdpi.org/sensors/papers/s8042722.pdf","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1424-8220","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Michikawa, Yuichi"}],"nameIdentifiers":[{"nameIdentifier":"448663","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suga, Tomo"}],"nameIdentifiers":[{"nameIdentifier":"448664","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohtsuka, Yoshimi"}],"nameIdentifiers":[{"nameIdentifier":"448665","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumoto, Izumi"}],"nameIdentifiers":[{"nameIdentifier":"448666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Atsuko"}],"nameIdentifiers":[{"nameIdentifier":"448667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Kenichi"}],"nameIdentifiers":[{"nameIdentifier":"448668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwakawa, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"448669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"448670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"道川 祐市","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448671","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅 智","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448672","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荘司 好美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448673","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 いづみ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 敦子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 顕一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448676","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩川 眞由美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"448678","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Visible genotype sensor array","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Visible genotype sensor array"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-05-27"},"publish_date":"2008-05-27","publish_status":"0","recid":"45172","relation_version_is_last":true,"title":["Visible genotype sensor array"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:06:49.026126+00:00"}