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Caffeine sensitizes nondividing human fibroblasts to X rays by inducing a high frequency of misrepair
https://repo.qst.go.jp/records/44731
https://repo.qst.go.jp/records/44731ecf3dd3d-6691-4c65-b766-b5b346f363b4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-11-30 | |||||
| タイトル | ||||||
| タイトル | Caffeine sensitizes nondividing human fibroblasts to X rays by inducing a high frequency of misrepair | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kawata, Tetsuya
× Kawata, Tetsuya× Ito, Hisao× Saitou, Masayoshi× Uno, Takashi× Okayasu, Ryuichi× et.al× 川田 哲也× 伊東 久夫× 斉藤 正好× 宇野 隆× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Caffeine sensitizes cells to ionizing radiation and this effect is believed to be associated with the disruption of DNA damage-responsive cell cycle checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining of dsbs is one of the underlying mechanisms of A-T cell hyper radiosensitivity. In this study, we investigated the effects of caffeine and radiation on non-growing G-0 phase normal human fibroblast cells by determining cell survival and scoring aberrations in calyculin-A induced G2 chromosomes. Results from the cell survival study indicate that after X-ray exposure G0 cells were sensitized by 24-h treatment with caffeine. Analysis of chromosome aberrations using FISH (fluorescence in situ hybridization) revealed a high frequency of aberrant cells and color-junctions in the caffeine treated cells. Since the majority of DNA repair in non-growing G-0 cells is believed to undergo non-homologous end joining (NHEJ), caffeine may influence the fidelity of NHEJ pathway in irradiated G-0 phase cells. | |||||
| 書誌情報 |
Radiation Research 巻 164, 号 4, p. 509-513, 発行日 2005 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
