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A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G1 phase
https://repo.qst.go.jp/records/44136
https://repo.qst.go.jp/records/4413674bf17e7-0c60-4a39-9276-6e314b50b326
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-02 | |||||
| タイトル | ||||||
| タイトル | A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G1 phase | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sugaya, Kimihiko
× Sugaya, Kimihiko× Sasanuma, Shunichi× Cook, Peter× Mita, Kazuei× 菅谷 公彦× 笹沼 俊一× 三田 和英 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Transcriptional activity of RNA polymerase II is modulated during the cell cycle. We previously identified a temperature-sensitive mutation in the largest (catalytic) subunit of RNA polymerase II (RPB1) that causes cell-cycle arrest and genome instability. We now characterize a different cell line that has a temperature-sensitive defect in cell cycle progression, and find that it also has a mutation in RPB1. The temperature-sensitive mutant, tsAF8, of the Syrian hamster cell line, BHK21, arrests at the non-permissive temperature in mid-G1 phase. We show that RPB1 in tsAF8 ? which is found exclusively in the nucleus at the permissive temperature ? is also found in the cytoplasm at the non-permissive temperature. Comparison of the DNA sequences of the RPB1 gene in the wild-type and mutant shows the mutant phenotype results from a (hemizygous) C-to-A variation at nucleotide 944 in one RPB1 allele; this gives rise to an ala-to-asp substitution at residue 315 in the protein. Aligning the amino acid sequences from various species reveals that ala315 is highly conserved in eukaryotes. | |||||
| 書誌情報 |
Gene 巻 274, p. 77-81, 発行日 2001 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0378-1119 | |||||
