{"created":"2023-05-15T14:34:03.593371+00:00","id":44136,"links":{},"metadata":{"_buckets":{"deposit":"2dc6a3c8-7f5c-4308-8cbe-7199ca0de1ab"},"_deposit":{"created_by":1,"id":"44136","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44136"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044136","sets":["1"]},"author_link":["438680","438679","438681","438678","438677","438682","438683"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"81","bibliographicPageStart":"77","bibliographicVolumeNumber":"274","bibliographic_titles":[{"bibliographic_title":"Gene"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transcriptional activity of RNA polymerase II is modulated during the cell cycle.  We previously identified a temperature-sensitive mutation in the largest (catalytic) subunit of RNA polymerase II (RPB1) that causes cell-cycle arrest and genome instability.  We now characterize a different cell line that has a temperature-sensitive defect in cell cycle progression, and find that it also has a mutation in RPB1.  The temperature-sensitive mutant, tsAF8, of the Syrian hamster cell line, BHK21, arrests at the non-permissive temperature in mid-G1 phase.  We show that RPB1 in tsAF8 ? which is found exclusively in the nucleus at the permissive temperature ? is also found in the cytoplasm at the non-permissive temperature.  Comparison of the DNA sequences of the RPB1 gene in the wild-type and mutant shows the mutant phenotype results from a (hemizygous) C-to-A variation at nucleotide 944 in one RPB1 allele; this gives rise to an ala-to-asp substitution at residue 315 in the protein.  Aligning the amino acid sequences from various species reveals that ala315 is highly conserved in eukaryotes.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0378-1119","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sugaya, Kimihiko"}],"nameIdentifiers":[{"nameIdentifier":"438677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasanuma, Shunichi"}],"nameIdentifiers":[{"nameIdentifier":"438678","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Cook, Peter"}],"nameIdentifiers":[{"nameIdentifier":"438679","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mita, Kazuei"}],"nameIdentifiers":[{"nameIdentifier":"438680","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅谷 公彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438681","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"笹沼 俊一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438682","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三田 和英","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"438683","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G1 phase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G1 phase"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-06-02"},"publish_date":"2006-06-02","publish_status":"0","recid":"44136","relation_version_is_last":true,"title":["A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G1 phase"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:18:58.843398+00:00"}