@article{oai:repo.qst.go.jp:00044136,
 author = {Sugaya, Kimihiko and Sasanuma, Shunichi and Cook, Peter and Mita, Kazuei and 菅谷 公彦 and 笹沼 俊一 and 三田 和英},
 journal = {Gene},
 month = {},
 note = {Transcriptional activity of RNA polymerase II is modulated during the cell cycle.  We previously identified a temperature-sensitive mutation in the largest (catalytic) subunit of RNA polymerase II (RPB1) that causes cell-cycle arrest and genome instability.  We now characterize a different cell line that has a temperature-sensitive defect in cell cycle progression, and find that it also has a mutation in RPB1.  The temperature-sensitive mutant, tsAF8, of the Syrian hamster cell line, BHK21, arrests at the non-permissive temperature in mid-G1 phase.  We show that RPB1 in tsAF8 ? which is found exclusively in the nucleus at the permissive temperature ? is also found in the cytoplasm at the non-permissive temperature.  Comparison of the DNA sequences of the RPB1 gene in the wild-type and mutant shows the mutant phenotype results from a (hemizygous) C-to-A variation at nucleotide 944 in one RPB1 allele; this gives rise to an ala-to-asp substitution at residue 315 in the protein.  Aligning the amino acid sequences from various species reveals that ala315 is highly conserved in eukaryotes.},
 pages = {77--81},
 title = {A mutation in the largest (catalytic) subunit of RNA polymerase II and its relation to the arrest of the cell cycle in G1 phase},
 volume = {274},
 year = {2001}
}