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Introduction of a foreign gene into medakafish using the particle gun method
https://repo.qst.go.jp/records/43111
https://repo.qst.go.jp/records/43111f522a3f0-ff73-4437-85ea-9afee62125d8
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-06-30 | |||||
| タイトル | ||||||
| タイトル | Introduction of a foreign gene into medakafish using the particle gun method | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamauchi, Masatake
× Yamauchi, Masatake× Kinoshita, Masato× Sasanuma, Motoe× Tsuji, Satsuki× Terada, Minako× Morimyo, Mitsuoki× Ishikawa, Yuuji× 山内 正剛× 辻 さつき× 寺田 美奈子× 森明 充興× 石川 裕二 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We developed a procedure to introduce a foreign gene into fertilized eggs of medakafish (Oryzias latipes) using the particle gun method, which is one of the easiest and most reliable techniques for gene transfer. A plasmid construct with the green fluorescence protein (GFP) gene driven by the medakafish beta-actin gene promoter was successfully introduced into eggs, and the expression of GFP was observed in 20% of the primary transfectant (chimera) fish. In addition, germ line transmission of GFP was observed in 13% of the GFP-positive primary transfectant fish. The new application described here should enable us to investigate gene expression using the fish model on a routine basis without high technical sophistication. |
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| 書誌情報 |
Journal of Experimental Zoology 巻 287, p. 285-293, 発行日 2000 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-104X | |||||
