@article{oai:repo.qst.go.jp:00043111,
 author = {Yamauchi, Masatake and Kinoshita, Masato and Sasanuma, Motoe and Tsuji, Satsuki and Terada, Minako and Morimyo, Mitsuoki and Ishikawa, Yuuji and 山内 正剛 and 辻 さつき and 寺田 美奈子 and 森明 充興 and 石川 裕二},
 journal = {Journal of Experimental Zoology},
 month = {},
 note = {We developed a procedure to introduce a foreign gene into fertilized eggs of
medakafish (Oryzias latipes) using the particle gun method, which is one of
the easiest and most reliable techniques for gene transfer.  A plasmid
construct with the green fluorescence protein (GFP) gene driven by the
medakafish beta-actin gene promoter was successfully introduced into eggs,
and the expression of GFP was observed in 20% of the primary transfectant
(chimera) fish.  In addition, germ line transmission of GFP was observed 
in 13% of the GFP-positive primary transfectant fish.  The new application 
described here should enable us to investigate gene expression using the
fish model on a routine basis without high technical sophistication.},
 pages = {285--293},
 title = {Introduction of a foreign gene into medakafish using the particle gun method},
 volume = {287},
 year = {2000}
}