{"created":"2023-05-15T14:33:14.901387+00:00","id":43111,"links":{},"metadata":{"_buckets":{"deposit":"52948909-d041-44ec-85a6-f0a00b00e24b"},"_deposit":{"created_by":1,"id":"43111","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43111"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043111","sets":["1"]},"author_link":["428933","428928","428939","428932","428937","428935","428936","428929","428938","428930","428931","428934"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"293","bibliographicPageStart":"285","bibliographicVolumeNumber":"287","bibliographic_titles":[{"bibliographic_title":"Journal of Experimental Zoology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We developed a procedure to introduce a foreign gene into fertilized eggs of\nmedakafish (Oryzias latipes) using the particle gun method, which is one of\nthe easiest and most reliable techniques for gene transfer.  A plasmid\nconstruct with the green fluorescence protein (GFP) gene driven by the\nmedakafish beta-actin gene promoter was successfully introduced into eggs,\nand the expression of GFP was observed in 20% of the primary transfectant\n(chimera) fish.  In addition, germ line transmission of GFP was observed \nin 13% of the GFP-positive primary transfectant fish.  The new application \ndescribed here should enable us to investigate gene expression using the\nfish model on a routine basis without high technical sophistication.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-104X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamauchi, Masatake"}],"nameIdentifiers":[{"nameIdentifier":"428928","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kinoshita, Masato"}],"nameIdentifiers":[{"nameIdentifier":"428929","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasanuma, Motoe"}],"nameIdentifiers":[{"nameIdentifier":"428930","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Satsuki"}],"nameIdentifiers":[{"nameIdentifier":"428931","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Terada, Minako"}],"nameIdentifiers":[{"nameIdentifier":"428932","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morimyo, Mitsuoki"}],"nameIdentifiers":[{"nameIdentifier":"428933","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikawa, Yuuji"}],"nameIdentifiers":[{"nameIdentifier":"428934","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山内 正剛","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428935","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 さつき","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428936","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田 美奈子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428937","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森明 充興","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428938","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 裕二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428939","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Introduction of a foreign gene into medakafish using the particle gun method","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Introduction of a foreign gene into medakafish using the particle gun method"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-06-30"},"publish_date":"2003-06-30","publish_status":"0","recid":"43111","relation_version_is_last":true,"title":["Introduction of a foreign gene into medakafish using the particle gun method"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:31:09.264025+00:00"}