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Heavy-ion-induced mutations in the gpt delta transgenic mouse: Effect of p53 gene knockout
https://repo.qst.go.jp/records/43007
https://repo.qst.go.jp/records/43007393305d8-a32a-443f-9670-e1656a6e53f5
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-11-15 | |||||
| タイトル | ||||||
| タイトル | Heavy-ion-induced mutations in the gpt delta transgenic mouse: Effect of p53 gene knockout | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yatagai, Fumio
× Yatagai, Fumio× Kurobe, Toshihiro× Nohmi, Takehiko× Ichi, Masumura Ken× Tsukada, Teruyo× Yamaguchi, Hirotake× Eguchi-Kasai, Kiyomi× Fukunishi, Nobuhisa× 谷田貝 文夫× 能美 健彦× 増村 健一× 笠井 清美 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The influence of the loss of p53 gene on heavy-ion-induced mutations was examined by constructing a new line of transgenic mice, p53 knockout (p53-/-) gpt delta. In this mouse model, deletions in lambda DNA integrated into the mouse genome are preferentially selected as Spi- phages, which can then be subjected to molecular analysis. Mice were exposed to 10 Gy of whole-body carbon-ion irradiation. The carbon ions were accelerated to 135 MeV/u by the RIKEN Ring Cyclotron. The p53 defect markedly enhanced the Spi- mutant frequency (MF) in the kidneys of mice exposed to C-ion irradiation: the Spi- MF increased 4.4- and 2.8-fold over the background level after irradiation in p53-/- and p53+/+ mice, respectively. There was no significant difference in the background Spi- MF between p53-/- and p53+/+ mice. Sequence analysis of the Spi- mutants indicated that the enhancement of kidney Spi- MF in p53-/- mice was primarily due to an increase in complex or rearranged-type deletions. In contrast to the kidney, the p53 defect had no effect on the Spi- MF in liver: Spi- MF increased 3.0- and 2.7-fold after the irradiation in p53-/- and p53+/+ mice, respectively. Our results suggest that p53 suppresses deletion mutations induced by heavy-ion irradiation in an organ-specific manner. | |||||
| 書誌情報 |
Environmental and Molecular Mutagenesis 巻 40, p. 216-225, 発行日 2002 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0893-6692 | |||||
