{"created":"2023-05-15T14:33:10.353846+00:00","id":43007,"links":{},"metadata":{"_buckets":{"deposit":"5b7b52ad-4623-4412-b924-2bb13e06b466"},"_deposit":{"created_by":1,"id":"43007","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"43007"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00043007","sets":["1"]},"author_link":["428002","428000","428004","428003","428001","428005","427998","427999","427996","427995","428006","427997"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"225","bibliographicPageStart":"216","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"Environmental and Molecular Mutagenesis"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The influence of the loss of p53 gene on heavy-ion-induced mutations was examined by constructing a new line of transgenic mice, p53 knockout (p53-/-) gpt delta. In this mouse model, deletions in lambda DNA integrated into the mouse genome are preferentially selected as Spi- phages, which can then be subjected to molecular analysis. Mice were exposed to 10 Gy of whole-body carbon-ion irradiation. The carbon ions were accelerated to 135 MeV/u by the RIKEN Ring Cyclotron. The p53 defect markedly enhanced the Spi- mutant frequency (MF) in the kidneys of mice exposed to C-ion irradiation: the Spi- MF increased 4.4- and 2.8-fold over the background level after irradiation in p53-/- and p53+/+ mice, respectively. There was no significant difference in the background Spi- MF between p53-/- and p53+/+ mice. Sequence analysis of the Spi- mutants indicated that the enhancement of kidney Spi- MF in p53-/- mice was primarily due to an increase in complex or rearranged-type deletions. In contrast to the kidney, the p53 defect had no effect on the Spi- MF in liver: Spi- MF increased 3.0- and 2.7-fold after the irradiation in p53-/- and p53+/+ mice, respectively. Our results suggest that p53 suppresses deletion mutations induced by heavy-ion irradiation in an organ-specific manner.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0893-6692","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yatagai, Fumio"}],"nameIdentifiers":[{"nameIdentifier":"427995","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kurobe, Toshihiro"}],"nameIdentifiers":[{"nameIdentifier":"427996","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nohmi, Takehiko"}],"nameIdentifiers":[{"nameIdentifier":"427997","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichi, Masumura Ken"}],"nameIdentifiers":[{"nameIdentifier":"427998","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsukada, Teruyo"}],"nameIdentifiers":[{"nameIdentifier":"427999","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamaguchi, Hirotake"}],"nameIdentifiers":[{"nameIdentifier":"428000","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Eguchi-Kasai, Kiyomi"}],"nameIdentifiers":[{"nameIdentifier":"428001","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukunishi, Nobuhisa"}],"nameIdentifiers":[{"nameIdentifier":"428002","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"谷田貝 文夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428003","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"能美 健彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428004","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"増村 健一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428005","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"笠井 清美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"428006","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Heavy-ion-induced mutations in the gpt delta transgenic mouse: Effect of p53 gene knockout","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Heavy-ion-induced mutations in the gpt delta transgenic mouse: Effect of p53 gene knockout"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-11-15"},"publish_date":"2002-11-15","publish_status":"0","recid":"43007","relation_version_is_last":true,"title":["Heavy-ion-induced mutations in the gpt delta transgenic mouse: Effect of p53 gene knockout"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:32:21.872665+00:00"}